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Eukaryotic Cell, August 2008, p. 1373-1386, Vol. 7, No. 8
1535-9778/08/$08.00+0     doi:10.1128/EC.00085-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Septins Stabilize Mitochondria in Tetrahymena thermophila{triangledown} ,{dagger}

D. Wloga,1 I. Strzyzewska-Jówko,2 J. Gaertig,1* and M. Jerka-Dziadosz2*

Department of Cellular Biology, University of Georgia, Athens, Georgia 30602-2607,1 Polish Academy of Sciences, M. Nencki Institute of Experimental Biology, Department of Cell Biology, 3 Pasteur Street, 02-093 Warsaw, Poland2

Received 8 March 2008/ Accepted 18 June 2008

We describe phylogenetic and functional studies of three septins in the free-living ciliate Tetrahymena thermophila. Both deletion and overproduction of septins led to vacuolization of mitochondria, destabilization of the nuclear envelope, and increased autophagy. All three green fluorescent protein-tagged septins localized to mitochondria. Specific septins localized to the outer mitochondrial membrane, to septa formed during mitochondrial scission, or to the mitochondrion-associated endoplasmic reticulum. The only other septins known to localize to mitochondria are human ARTS and murine M-septin, both alternatively spliced forms of Sep4 (S. Larisch, Cell Cycle 3:1021-1023, 2004; S. Takahashi, R. Inatome, H. Yamamura, and S. Yanagi, Genes Cells 8:81-93, 2003). It therefore appears that septins have been recruited to mitochondrial functions independently in at least two eukaryotic lineages and in both cases are involved in apoptotic events.

* Corresponding author. Mailing address for J. Gaertig: Department of Cellular Biology, University of Georgia, Athens, GA 30602-2607. Phone: (706) 542-3409. Fax: 48 22 822 53 42. E-mail: jgaertig{at}cb.uga.edu. Mailing address for M. Jerka-Dziadosz: Polish Academy of Sciences, M. Nencki Institute of Experimental Biology, Department of Cell Biology, 3 Pasteur Street, 02-093 Warsaw, Poland. Phone: 48 22 5892 233. Fax: (706) 542-4271. E-mail: dziadosz{at}nencki.gov.pl

{triangledown} Published ahead of print on 27 June 2008.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

Eukaryotic Cell, August 2008, p. 1373-1386, Vol. 7, No. 8
1535-9778/08/$08.00+0     doi:10.1128/EC.00085-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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