Eukaryotic Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Other Versions of this Article:
EC.00036-08v1
7/7/1109    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Shi, L.
Right arrow Articles by Nosanchuk, J. D.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shi, L.
Right arrow Articles by Nosanchuk, J. D.

 Previous Article  |  Next Article 

Eukaryotic Cell, July 2008, p. 1109-1117, Vol. 7, No. 7
1535-9778/08/$08.00+0     doi:10.1128/EC.00036-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

A Monoclonal Antibody to Histoplasma capsulatum Alters the Intracellular Fate of the Fungus in Murine Macrophages{triangledown} ,{dagger}

Li Shi,1 Priscila C. Albuquerque,1 Eszter Lazar-Molnar,2 Xintao Wang,3 Laura Santambrogio,3 Attila Gácser,1,{ddagger} and Joshua D. Nosanchuk1,2*

Departments of Medicine (Division of Infectious Diseases),1 Microbiology & Immunology,2 Pathology, Albert Einstein College of Medicine, Bronx, New York3

Received 30 January 2008/ Accepted 15 April 2008

Monoclonal antibodies (MAbs) to a cell surface histone on Histoplasma capsulatum modify murine infection and decrease the growth of H. capsulatum within macrophages. Without the MAbs, H. capsulatum survives within macrophages by modifying the intraphagosomal environment. In the present study, we aimed to analyze the affects of a MAb on macrophage phagosomes. Using transmission electron and fluorescence microscopy, we showed that phagosome activation and maturation are significantly greater when H. capsulatum yeast are opsonized with MAb. The MAb reduced the ability of the organism to regulate the phagosomal pH. Additionally, increased antigen processing and reduced negative costimulation occur in macrophages that phagocytose yeast cells opsonized with MAb, resulting in more-efficient T-cell activation. The MAb alters the intracellular fate of H. capsulatum by affecting the ability of the fungus to regulate the milieu of the phagosome.

* Corresponding author. Mailing address: Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461. Phone: (718) 430-2366. Fax: (718) 430-8968. E-mail: nosanchu{at}aecom.yu.edu

{triangledown} Published ahead of print on 16 May 2008.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

{ddagger} Present address: Department of Microbiology, University of Szeged, Szeged, Hungary.

Eukaryotic Cell, July 2008, p. 1109-1117, Vol. 7, No. 7
1535-9778/08/$08.00+0     doi:10.1128/EC.00036-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Appl. Environ. Microbiol. Infect. Immun. J. Bacteriol.
Mol. Cell Biol. Microbiol. Mol. Biol. Rev. ALL ASM JOURNALS
Copyright © 2008 by the American Society for Microbiology.