This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Helmstaedt, K.
Right arrow Articles by Braus, G. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Helmstaedt, K.
Right arrow Articles by Braus, G. H.

 Previous Article  |  Next Article 

Eukaryotic Cell, June 2008, p. 1041-1052, Vol. 7, No. 6
1535-9778/08/$08.00+0     doi:10.1128/EC.00071-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

The Nuclear Migration Protein NUDF/LIS1 Forms a Complex with NUDC and BNFA at Spindle Pole Bodies{triangledown}

Kerstin Helmstaedt,1,2 Karen Laubinger,1,2 Katja Voßkuhl,1,{dagger} Özgür Bayram,1 Silke Busch,1,{ddagger} Michael Hoppert,3 Oliver Valerius,1 Stephan Seiler,1,2* and Gerhard H. Braus1*

Molekulare Mikrobiologie und Genetik, Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Grisebachstraße 8, D-37077 Göttingen, Germany,1 DFG Research Center for Molecular Physiology of the Brain (CMPB), D-37077 Göttingen, Germany;,2 Allgemeine Mikrobiologie, Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Grisebachstraße 8, D-37077 Göttingen, Germany3

Received 8 March 2007/ Accepted 25 March 2008

Nuclear migration depends on microtubules, the dynein motor complex, and regulatory components like LIS1 and NUDC. We sought to identify new binding partners of the fungal LIS1 homolog NUDF to clarify its function in dynein regulation. We therefore analyzed the association between NUDF and NUDC in Aspergillus nidulans. NUDF and NUDC directly interacted in yeast two-hybrid experiments via NUDF's WD40 domain. NUDC-green fluorescent protein (NUDC-GFP) was localized to immobile dots in the cytoplasm and at the hyphal cortex, some of which were spindle pole bodies (SPBs). We showed by bimolecular fluorescence complementation microscopy that NUDC directly interacted with NUDF at SPBs at different stages of the cell cycle. Applying tandem affinity purification, we isolated the NUDF-associated protein BNFA (for binding to NUDF). BNFA was dispensable for growth and for nuclear migration. GFP-BNFA fusions localized to SPBs at different stages of the cell cycle. This localization depended on NUDF, since the loss of NUDF resulted in the cytoplasmic accumulation of BNFA. BNFA did not bind to NUDC in a yeast two-hybrid assay. These results show that the conserved NUDF and NUDC proteins play a concerted role at SPBs at different stages of the cell cycle and that NUDF recruits additional proteins specifically to the dynein complex at SPBs.

* Corresponding author. Mailing address: Molekulare Mikrobiologie und Genetik, Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Grisebachstraβe 8, D-37077 Göttingen, Germany. Phone: 49-551-393771. Fax: 49-551-393820. E-mail for S. Seiler: sseiler{at}gwdg.de. E-mail for G. H. Braus: gbraus{at}gwdg.de

{triangledown} Published ahead of print on 4 April 2008.

{dagger} Present address: Abteilung Gastroenterologie, Hepatologie und Endokrinologie, Medizinische Hochschule Hannover, Carl-Neuberg-Straβe 1, D-30625 Hannover, Germany.

{ddagger} Present address: Zentrum für Arzneimittelforschung, Entwicklung und Sicherheit (ZAFES), Institut für Klinische Pharmakologie, Klinikum der Johann Wolfgang Goethe-Universität Frankfurt, Theodor-Stern-Kai 7, D-60590 Frankfurt am Main, Germany.

Eukaryotic Cell, June 2008, p. 1041-1052, Vol. 7, No. 6
1535-9778/08/$08.00+0     doi:10.1128/EC.00071-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jurkuta, R. J., Kaplinsky, N. J., Spindel, J. E., Barton, M. K. (2009). Partitioning the Apical Domain of the Arabidopsis Embryo Requires the BOBBER1 NudC Domain Protein. Plant Cell 21: 1957-1971 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»