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Eukaryotic Cell, June 2008, p. 1019-1028, Vol. 7, No. 6
1535-9778/08/$08.00+0     doi:10.1128/EC.00413-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Molecular Signals in the Trafficking of Toxoplasma gondii Protein MIC3 to the Micronemes{triangledown}

Hiba El Hajj,1 Julien Papoin,1 Odile Cérède,3 Nathalie Garcia-Réguet,2 Martine Soête,2 Jean-François Dubremetz,1 and Maryse Lebrun1*

UMR 5235 CNRS, Université de Montpellier 2, CP 107, Place Eugène Bataillon, 34090 Montpellier, France,1 FRE 2377 CNRS, Institut de Biologie de Lille, 1 rue du Professeur Calmette, 59021 Lille, France,2 UMR Université-INRA d'Immunologie Parasitaires, Faculté des Sciences Pharmaceutiques et Biologiques, 31 Avenue Monge, 37200 Tours, France3

Received 9 November 2007/ Accepted 26 February 2008

The protozoan parasite Toxoplasma gondii is equipped with a sophisticated secretory apparatus, including three distinct exocytic organelles, named micronemes, rhoptries, and dense granules. We have dissected the requirements for targeting the microneme protein MIC3, a key component of T. gondii infection. We have shown that MIC3 is processed in a post-Golgi compartment and that the MIC3 propeptide and epidermal growth factor (EGF) modules contain microneme-targeting information. The minimal requirement for microneme delivery is defined by the propeptide plus any one of the three EGF domains. We have demonstrated that the cleavage of the propeptide, the dimerization of MIC3, and the chitin binding-like sequence, which are crucial for host cell binding and virulence, are dispensable for proper targeting. Finally, we have shown that part of MIC3 is withheld in the secretory pathway in a cell cycle-dependent manner.

* Corresponding author. Mailing address: UMR 5235 CNRS, Université de Montpellier 2, CP 107, Place Eugène Bataillon, 34090 Montpellier, France. Phone: 33 467 143 455. Fax: 33 467 144 286. E-mail: maryse.lebrun{at}univ-montp2.fr

{triangledown} Published ahead of print on 4 April 2008.

Eukaryotic Cell, June 2008, p. 1019-1028, Vol. 7, No. 6
1535-9778/08/$08.00+0     doi:10.1128/EC.00413-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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