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Eukaryotic Cell, November 2008, p. 2004-2007, Vol. 7, No. 11
1535-9778/08/$08.00+0     doi:10.1128/EC.00142-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Inducible RNA Interference of brlAβ in Aspergillus nidulans{triangledown}

L. M. Barton and R. A. Prade*

Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, Oklahoma 74078

Received 22 April 2008/ Accepted 20 August 2008

An inducible RNA interference (RNAi) construct composed of inverted repeating alcA promoters flanking the developmental regulatory gene brlAβ was tested in Aspergillus nidulans. On inducing medium, the RNAi strains failed to sporulate and lacked brlA{alpha} and brlAβ expression. RNAi was specific for brlAβ, but not brlA{alpha}, silencing, indicating brlA{alpha} regulation by brlAβ.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, Oklahoma State University, 307 LSE, Stillwater, OK 74078. Phone: (405) 744-5794. Fax: (405) 744-6790. E-mail: prade{at}okstate.edu

{triangledown} Published ahead of print on 29 August 2008.

Eukaryotic Cell, November 2008, p. 2004-2007, Vol. 7, No. 11
1535-9778/08/$08.00+0     doi:10.1128/EC.00142-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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