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Eukaryotic Cell, November 2008, p. 1965-1979, Vol. 7, No. 11
1535-9778/08/$08.00+0     doi:10.1128/EC.00418-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Transcriptome for Photobiological Hydrogen Production Induced by Sulfur Deprivation in the Green Alga Chlamydomonas reinhardtii ^,

Anh Vu Nguyen,^1,3, Skye R. Thomas-Hall,^1, Alizée Malnoë,² Matthew Timmins,¹ Jan H. Mussgnug,^2,3 Jens Rupprecht,² Olaf Kruse,³ Ben Hankamer,^1,2 and Peer M. Schenk^1*

School of Integrative Biology,¹ Institute for Molecular Bioscience, University of Queensland, St. Lucia, Queensland 4072, Australia,² Department of Biology/AlgaeBioTech Group, University of Bielefeld, Bielefeld, Germany³

Received 15 November 2007/ Accepted 5 August 2008

Photobiological hydrogen production using microalgae is being developed into a promising clean fuel stream for the future. In this study, microarray analyses were used to obtain global expression profiles of mRNA abundance in the green alga Chlamydomonas reinhardtii at different time points before the onset and during the course of sulfur-depleted hydrogen production. These studies were followed by real-time quantitative reverse transcription-PCR and protein analyses. The present work provides new insights into photosynthesis, sulfur acquisition strategies, and carbon metabolism-related gene expression during sulfur-induced hydrogen production. A general trend toward repression of transcripts encoding photosynthetic genes was observed. In contrast to all other LHCBM genes, the abundance of the LHCBM9 transcript (encoding a major light-harvesting polypeptide) and its protein was strongly elevated throughout the experiment. This suggests a major remodeling of the photosystem II light-harvesting complex as well as an important function of LHCBM9 under sulfur starvation and photobiological hydrogen production. This paper presents the first global transcriptional analysis of C. reinhardtii before, during, and after photobiological hydrogen production under sulfur deprivation.

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* Corresponding author. Mailing address: School of Integrative Biology, University of Queensland, St. Lucia, Queensland 4072, Australia. Phone: 61-7-33658817. Fax: 61-7-33651699. E-mail: p.schenk{at}uq.edu.au

Published ahead of print on 15 August 2008.

Supplemental material for this article may be found at http://ec.asm.org/.

These authors contributed equally.

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Eukaryotic Cell, November 2008, p. 1965-1979, Vol. 7, No. 11
1535-9778/08/$08.00+0     doi:10.1128/EC.00418-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

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