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 Previous Article

Eukaryotic Cell, October 2008, p. 1865-1873, Vol. 7, No. 10
1535-9778/08/$08.00+0     doi:10.1128/EC.00125-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Septal Pore Cap Protein SPC18, Isolated from the Basidiomycetous Fungus Rhizoctonia solani, Also Resides in Pore Plugs{triangledown}

Kenneth G. A. van Driel,1,{dagger} Arend F. van Peer,2 Jan Grijpstra,2 Han A. B. Wösten,2 Arie J. Verkleij,3 Wally H. Müller,3 and Teun Boekhout1*

CBS Fungal Biodiversity Centre, Institute of the Royal Netherlands Academy of Arts and Sciences (KNAW), Uppsalalaan 8, 3584 CT Utrecht, The Netherlands,1 Molecular Microbiology, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands,2 Cellular Architecture and Dynamics, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands3

Received 10 April 2008/ Accepted 19 August 2008

The hyphae of filamentous fungi are compartmentalized by septa that have a central pore. The fungal septa and septum-associated structures play an important role in maintaining cellular and intrahyphal homeostasis. The dolipore septa in the higher Basidiomycota (i.e., Agaricomycotina) are associated with septal pore caps. Although the ultrastructure of the septal pore caps has been studied extensively, neither the biochemical composition nor the function of these organelles is known. Here, we report the identification of the glycoprotein SPC18 that was found in the septal pore cap-enriched fraction of the basidiomycetous fungus Rhizoctonia solani. Based on its N-terminal sequence, the SPC18 gene was isolated. SPC18 encodes a protein of 158 amino acid residues, which contains a hydrophobic signal peptide for targeting to the endoplasmic reticulum and has an N-glycosylation motif. Immunolocalization showed that SPC18 is present in the septal pore caps. Surprisingly, we also observed SPC18 being localized in some plugs. The data reported here strongly support the hypothesis that septal pore caps are derived from endoplasmic reticulum and are involved in dolipore plugging and, thus, contribute to hyphal homeostasis in basidiomycetous fungi.

* Corresponding author. Mailing address: CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands. Phone: 31 (0) 30 2122671. Fax: 31 (0) 30 2512097. E-mail: t.boekhout{at}cbs.knaw.nl

{triangledown} Published ahead of print on 29 August 2008.

{dagger} Present address: Mahidol University, Faculty of Science, Department of Microbiology, Rama VI Road, Bangkok, Thailand.

Eukaryotic Cell, October 2008, p. 1865-1873, Vol. 7, No. 10
1535-9778/08/$08.00+0     doi:10.1128/EC.00125-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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