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Eukaryotic Cell, August 2007, p. 1363-1372, Vol. 6, No. 8
1535-9778/07/$08.00+0     doi:10.1128/EC.00165-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Alteration of the Protein Kinase Binding Domain Enhances Function of the Saccharomyces cerevisiae Molecular Chaperone Cdc37{triangledown}

Min Ren,1,2 Arti Santhanam,1,2 Paul Lee,3 Avrom Caplan,3 and Stephen Garrett2*

Graduate School of Biomedical Sciences,1 Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, Newark, New Jersey 07101,2 Department of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine, New York, New York 100293

Received 8 May 2007/ Accepted 2 June 2007

Cdc37 is a molecular chaperone that has a general function in the biogenesis of protein kinases. We identified mutations within the putative "protein kinase binding domain" of Cdc37 that alleviate the conditional growth defect of a strain containing a temperature-sensitive allele, tpk2(Ts), of the cyclic AMP-dependent protein kinase (PKA). These dominant mutations alleviate the temperature-sensitive growth defect by elevating PKA activity, as judged by their effects on PKA-regulated processes, localization and phosphorylation of the PKA effector Msn2, as well as in vitro PKA activity. Although the tpk2(Ts) growth defect is also alleviated by Cdc37 overproduction, the CDC37 dominant mutants contain wild-type Cdc37 protein levels. In addition, Saccharomyces cerevisiae Ste11 protein kinase has an elevated physical interaction with the altered Cdc37 protein. These results implicate specific amino-terminal residues in the interaction between Cdc37 and client protein kinases and provide further genetic and biochemical support for a model in which Cdc37 functions as a molecular chaperone for protein kinases.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, 225 Warren St., Newark, NJ 07101. Phone: (973) 972-4483, ext. 20698. Fax: (973) 972-8982. E-mail: garretst{at}umdnj.edu

{triangledown} Published ahead of print on 15 June 2007.

Eukaryotic Cell, August 2007, p. 1363-1372, Vol. 6, No. 8
1535-9778/07/$08.00+0     doi:10.1128/EC.00165-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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