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Eukaryotic Cell, August 2007, p. 1310-1319, Vol. 6, No. 8
1535-9778/07/$08.00+0     doi:10.1128/EC.00136-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Development of a Gene Knockout System in Candida parapsilosis Reveals a Conserved Role for BCR1 in Biofilm Formation{triangledown} ,{dagger}

Chen Ding and Geraldine Butler*

UCD School of Biomolecular and Biomedical Science, Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland

Received 23 April 2007/ Accepted 7 June 2007

Candida parapsilosis is an important cause of candidiasis, yet few molecular tools are available. We adapted a recyclable nourseothricin resistance marker gene (SAT1) originally developed for use with C. albicans in order to generate gene knockouts from C. parapsilosis. We first replaced the promoters driving expression of the FLP recombinase and the SAT1 genes with the equivalent sequences from C. parapsilosis. We then used the cassette to generate a homozygous knockout of C. parapsilosis URA3. The ura3 knockouts have altered colony morphologies. We also knocked out both alleles of an ortholog of BCR1, a gene encoding a transcription factor known to be required for biofilm development in C. albicans. We show that C. parapsilosis BCR1 is necessary for biofilm development in C. parapsilosis and for expression of the cell wall protein encoded by RBT1. Our results suggest that there are significant similarities in the regulation of biofilms between the two species, despite the fact that C. parapsilosis does not generate true hyphae and that BCR1 regulates the expression of many hypha-specific adhesins in C. albicans.

* Corresponding author. Mailing address: UCD School of Biomolecular and Biomedical Science, Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland. Phone: 353-1-7166885. Fax: 353-1-2837211. E-mail: geraldine.butler{at}ucd.ie

{triangledown} Published ahead of print on 22 June 2007.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

Eukaryotic Cell, August 2007, p. 1310-1319, Vol. 6, No. 8
1535-9778/07/$08.00+0     doi:10.1128/EC.00136-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Rossignol, T., Ding, C., Guida, A., d'Enfert, C., Higgins, D. G., Butler, G. (2009). Correlation between Biofilm Formation and the Hypoxic Response in Candida parapsilosis. Eukaryot Cell 8: 550-559 [Abstract] [Full Text]  
  • Trofa, D., Gacser, A., Nosanchuk, J. D. (2008). Candida parapsilosis, an Emerging Fungal Pathogen. Clin. Microbiol. Rev. 21: 606-625 [Abstract] [Full Text]  


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