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 Previous Article

Eukaryotic Cell, July 2007, p. 1248-1250, Vol. 6, No. 7
1535-9778/07/$08.00+0     doi:10.1128/EC.00110-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

RNA Interference Mutant Induction In Vivo Demonstrates the Essential Nature of Trypanosome Flagellar Function during Mammalian Infection{triangledown}

Samantha Griffiths, Neil Portman, Philip R. Taylor,{dagger} Siamon Gordon, Michael L. Ginger, and Keith Gull*

Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom

Received 5 April 2007/ Accepted 7 May 2007

We demonstrate that trypanosomes compromised in flagellar function are rapidly cleared from infected mice. Analysis of the PFR2 bloodstream RNA interference mutant revealed that defective cell motility occurred prior to cytokinesis failure. This validation provides a paradigm for the flagellum as a target for future assays and interventions against this human pathogen.

* Corresponding author. Mailing address: Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom. Phone: 44 (0) 1865 285752. Fax: 44 (0) 1865 285691. E-mail: keith.gull{at}path.ox.ac.uk

{triangledown} Published ahead of print on 18 May 2007.

{dagger} Present address: Department of Medical Biochemistry and Immunology, School of Medicine, Tenovus Building, Heath Campus, Cardiff, CF14 4XN, United Kingdom.

Eukaryotic Cell, July 2007, p. 1248-1250, Vol. 6, No. 7
1535-9778/07/$08.00+0     doi:10.1128/EC.00110-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Portman, N., Lacomble, S., Thomas, B., McKean, P. G., Gull, K. (2009). Combining RNA Interference Mutants and Comparative Proteomics to Identify Protein Components and Dependences in a Eukaryotic Flagellum. J. Biol. Chem. 284: 5610-5619 [Abstract] [Full Text]  


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