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Eukaryotic Cell, June 2007, p. 997-1005, Vol. 6, No. 6
1535-9778/07/$08.00+0     doi:10.1128/EC.00011-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Involvement of a Magnaporthe grisea Serine/Threonine Kinase Gene, MgATG1, in Appressorium Turgor and Pathogenesis{triangledown}

Xiao-Hong Liu,1,2,{dagger} Jian-Ping Lu,2,{dagger} Lei Zhang,2 Bo Dong,1 Hang Min,2 and Fu-Cheng Lin1*

Biotechnology Institute, Zhejiang University, Kaixuan Road 268, Hangzhou 310029, China,1 College of Life Sciences, Zhejiang University, Yuhangtang Road 388, Hangzhou 310058, China2

Received 9 January 2007/ Accepted 29 March 2007

We isolated an MgATG1 gene encoding a serine/threonine protein kinase from the rice blast fungus Magnaporthe grisea. In the {Delta}Mgatg1 mutant, in which the MgATG1 gene had been deleted, autophagy was blocked; the mutant also showed fewer lipid droplets in its conidia, lower turgor pressure of the appressorium, and such defects in morphogenesis as delayed initiation and slower germination of conidia. As a result of lower turgor pressure of the appressorium, the {Delta}Mgatg1 mutant lost its ability to penetrate and infect the two host plants, namely, rice and barley. However, normal values of the parameters and infective abilities were restored on reintroducing an intact copy of the MgATG1 gene into the mutant. Autophagy is thus necessary for turnover of organic matter during the formation of conidia and appressoria and for normal development and pathogenicity in M. grisea.

* Corresponding author. Mailing address: Biotechnology Institute, Zhejiang University, Kaixuan Road 268, Hangzhou 310029, China. Phone: (86) 571 86971185. Fax: (86) 571 86971516. E-mail: fuchenglin{at}zju.edu.cn

{triangledown} Published ahead of print on 6 April 2007.

{dagger} X.-H.L. and J.-P.L. contributed equally to this work.

Eukaryotic Cell, June 2007, p. 997-1005, Vol. 6, No. 6
1535-9778/07/$08.00+0     doi:10.1128/EC.00011-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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