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Eukaryotic Cell, June 2007, p. 971-983, Vol. 6, No. 6
1535-9778/07/$08.00+0     doi:10.1128/EC.00016-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Mcp4, a Meiotic Coiled-Coil Protein, Plays a Role in F-Actin Positioning during Schizosaccharomyces pombe Meiosis{triangledown}

Ayami Ohtaka, Daisuke Okuzaki, Takamune T. Saito,{dagger} and Hiroshi Nojima*

Department of Molecular Genetics, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita City, Osaka 565-0871, Japan

Received 17 January 2007/ Accepted 26 March 2007

Some meiosis-specific proteins of Schizosaccharomyces pombe harbor coiled-coil motifs and play essential roles in meiotic progression. Here we describe Mcp4, a novel meiosis-specific protein whose expression is abruptly induced at the horsetail phase and which remains expressed until sporulation is finished. Fluorescence microscopic analysis revealed that Mcp4 alters its subcellular localization during meiosis in a manner that partially resembles the movement of F-actin during meiosis. Mcp4 and F-actin never colocalize; rather, they are located in a side-by-side manner. When forespore membrane formation begins at metaphase II, the Mcp4 signals assemble at the lagging face of the dividing nuclei. At this stage, they are sandwiched between F-actin and the nucleus. Mcp4, in turn, appears to sandwich F-actin with Meu14. In mcp4{Delta} cells at anaphase II, the F-actin, which is normally dumbbell-shaped, adopts an abnormal balloon shape. Spores of mcp4{Delta} cells were sensitive to NaCl, although their shape and viability were normal. Taken together, we conclude that Mcp4 plays a role in the accurate positioning of F-actin during S. pombe meiosis.

* Corresponding author. Mailing address: Department of Molecular Genetics, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita City, Osaka 565-0871, Japan. Phone: 81-6-6875-3980. Fax: 81-6-6875-5192. E-mail: snj-0212{at}biken.osaka-u.ac.jp

{triangledown} Published ahead of print on 13 April 2007.

{dagger} Present address: Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, New Research Building, Room 334, Boston, MA 20115.

Eukaryotic Cell, June 2007, p. 971-983, Vol. 6, No. 6
1535-9778/07/$08.00+0     doi:10.1128/EC.00016-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nakamura, T., Asakawa, H., Nakase, Y., Kashiwazaki, J., Hiraoka, Y., Shimoda, C. (2008). Live Observation of Forespore Membrane Formation in Fission Yeast. Mol. Biol. Cell 19: 3544-3553 [Abstract] [Full Text]  
  • Ohtaka, A., Okuzaki, D., Nojima, H. (2008). Mug27 is a meiosis-specific protein kinase that functions in fission yeast meiosis II and sporulation. J. Cell Sci. 121: 1547-1558 [Abstract] [Full Text]  


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