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Eukaryotic Cell, June 2007, p. 1030-1040, Vol. 6, No. 6
1535-9778/07/$08.00+0     doi:10.1128/EC.00035-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Evidence that Noncoding RNA dutA Is a Multicopy Suppressor of Dictyostelium discoideum STAT Protein Dd-STATa{triangledown}

Nao Shimada and Takefumi Kawata*

Department of Biology, Faculty of Science, Toho University, Funabashi, Japan

Received 27 January 2007/ Accepted 3 April 2007

Dd-STATa, a Dictyostelium discoideum homologue of metazoan STAT transcription factors, is necessary for culmination. We created a mutant strain with partial Dd-STATa activity and used it to screen for unlinked suppressor genes. We screened approximately 450,000 clones from a slug-stage cDNA library for their ability to rescue the culmination defect when overexpressed. There were 12 multicopy suppressors of Dd-STATa, of which 4 encoded segments of a known noncoding RNA, dutA. Expression of dutA is specific to the pstA zone, the region where Dd-STATa is activated. In suppressed strains the expression patterns of several putative Dd-STATa target genes become similar to the wild-type strain. In addition, the amount of the tyrosine-phosphorylated form of Dd-STATa is significantly increased in the suppressed strain. These results indicate that partial copies of dutA may act upstream of Dd-STATa to regulate tyrosine phosphorylation by an unknown mechanism.

* Corresponding author. Mailing address: Department of Biology, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan. Phone and fax: 81-47-472-5156. E-mail: tkawata{at}bio.sci.toho-u.ac.jp

{triangledown} Published ahead of print on 13 April 2007.

Eukaryotic Cell, June 2007, p. 1030-1040, Vol. 6, No. 6
1535-9778/07/$08.00+0     doi:10.1128/EC.00035-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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