Eukaryotic Cell
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Eukaryotic Cell, April 2007, p. 664-673, Vol. 6, No. 4
1535-9778/07/$08.00+0     doi:10.1128/EC.00308-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

The 29-Kilodalton Thiol-Dependent Peroxidase of Entamoeba histolytica Is a Factor Involved in Pathogenesis and Survival of the Parasite during Oxidative Stress{triangledown}

Abhik Sen,1 Nabendu Sekhar Chatterjee,2 M. Ali Akbar,1,{dagger} Nilay Nandi,1 and Pradeep Das1*

Division of Microbiology, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road, Scheme-XM, Beliaghata, Kolkata-700 010, India,1 Division Of Biochemistry, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road, Scheme-XM, Beliaghata, Kolkata-700 010, India2

Received 27 September 2006/ Accepted 5 February 2007

The 29-kDa surface antigen (thiol-dependent peroxidase; Eh29) of Entamoeba histolytica exhibits peroxidative and protective antioxidant activities. During tissue invasion, the trophozoites are exposed to oxidative stress and need to deal with highly toxic reactive oxygen species (ROS). In this investigation, attempts have been made to understand the role of the 29-kDa peroxidase gene in parasite survival and pathogenesis. Inhibition of eh29 gene expression by antisense RNA technology has shown approximately 55% inhibition in eh29 expression, maximum ROS accumulation, and significantly lower viability in 29-kDa downregulated trophozoites during oxidative stress. The cytopathic and cytotoxic activities were also found to decrease effectively in the 29-kDa downregulated trophozoites. Size of liver abscesses was substantially lower in hamsters inoculated with 29-kDa downregulated trophozoites compared to the normal HM1:IMSS. These findings clearly suggest that the 29-kDa protein of E. histolytica has a role in both survival of trophozoites in the presence of ROS and pathogenesis of amoebiasis.

* Corresponding author. Present address: Rajendra Memorial Research Institute of Medical Sciences, Agam Kuan, Patna 800 007, India. Phone: 91 612 2636651. Fax: 91 612 2634379. E-mail: drpradeep.das{at}gmail.com

{triangledown} Published ahead of print on 16 February 2007.

{dagger} Present address: Southwestern Medical Center, University of Texas, Dallas, TX.

Eukaryotic Cell, April 2007, p. 664-673, Vol. 6, No. 4
1535-9778/07/$08.00+0     doi:10.1128/EC.00308-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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