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Eukaryotic Cell, March 2007, p. 563-567, Vol. 6, No. 3
1535-9778/07/$08.00+0     doi:10.1128/EC.00301-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Novel Method for Preparing Spheroplasts from Cells with an Internal Cellulosic Cell Wall{triangledown}

Alvin C. M. Kwok, Carmen C. M. Mak, Francis T. W. Wong, and Joseph T. Y. Wong*

Department of Biology, Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong SAR, People's Republic of China

Received 18 September 2006/ Accepted 12 January 2007

Protoplast and spheroplast preparations allow the transfer of macromolecules into cells and provide the basis for the generation of engineered organisms. Crypthecodinium cohnii cells harvested from polyethylene glycol-containing agar plates possessed significantly lower levels of cellulose in their cortical layers, which facilitated the delivery of fluorescence-labeled oligonucleotides into these cells.

* Corresponding author. Mailing address: Department of Biology, Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong SAR, People's Republic of China. Phone: 852-23587343. Fax: 852-23581559. E-mail: botin{at}ust.hk.

{triangledown} Published ahead of print on 26 January 2007.

Eukaryotic Cell, March 2007, p. 563-567, Vol. 6, No. 3
1535-9778/07/$08.00+0     doi:10.1128/EC.00301-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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