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Eukaryotic Cell, March 2007, p. 555-562, Vol. 6, No. 3
1535-9778/07/$08.00+0     doi:10.1128/EC.00266-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Aspergillus nidulans Dis1/XMAP215 Protein AlpA Localizes to Spindle Pole Bodies and Microtubule Plus Ends and Contributes to Growth Directionality{triangledown} ,{dagger}

Cathrin Enke,1,2,{ddagger} Nadine Zekert,1,{ddagger} Daniel Veith,1,2,{ddagger} Carolin Schaaf,1 Sven Konzack,1,2 and Reinhard Fischer1,2*

University of Karlsruhe, Applied Microbiology, Hertzstrasse 16, D-76187 Karlsruhe,1 and Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Str., D-35043 Marburg, Germany2

Received 18 August 2006/ Accepted 21 December 2006

The dynamics of cytoplasmic microtubules (MTs) is largely controlled by a protein complex at the MT plus end. In Schizosaccharomyces pombe and in filamentous fungi, MT plus end-associated proteins also determine growth directionality. We have characterized the Dis1/XMAP215 family protein AlpA from Aspergillus nidulans and show that it determines MT dynamics as well as hyphal morphology. Green fluorescent protein-tagged AlpA localized to MT-organizing centers (centrosomes) and to MT plus ends. The latter accumulation occurred independently of conventional kinesin or the Kip2-familiy kinesin KipA. alpA deletion strains were viable and only slightly temperature sensitive. Mitosis, nuclear migration, and nuclear positioning were not affected, but hyphae grew in curves rather than straight, which appeared to be an effect of reduced MT growth and dynamics.

* Corresponding author. Mailing address: University of Karlsruhe, Applied Microbiology, Hertzstrasse 16, D-76187 Karlsruhe. Phone: 49 721 608 4630. Fax: 49 721 608 4509. E-mail: reinhard.fischer{at}bio.uni-karlsruhe.de.

{triangledown} Published ahead of print on 19 January 2007.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

{ddagger} These authors contributed equally.

Eukaryotic Cell, March 2007, p. 555-562, Vol. 6, No. 3
1535-9778/07/$08.00+0     doi:10.1128/EC.00266-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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