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Transcriptional Activation Domains of the Candida albicans Gcn4p and Gal4p Homologs ^,

Department of Biology, McGill University, Stewart Biology Building, 1205 Avenue Docteur Penfield, Montreal, Quebec, Canada H3A 1B1,¹ Genetics Group, Biotechnology Research Institute, National Research Council, Montreal, Quebec, Canada H4P 2R2²

Received 14 June 2006/ Accepted 28 November 2006

Many putative transcription factors in the pathogenic fungus Candida albicans contain sequence similarity to well-defined transcriptional regulators in the budding yeast Saccharomyces cerevisiae, but this sequence similarity is often limited to the DNA binding domains of the molecules. The Gcn4p and Gal4p proteins of Saccharomyces cerevisiae are highly studied and well-understood eukaryotic transcription factors of the basic leucine zipper (Gcn4p) and C₆ zinc cluster (Gal4p) families; C. albicans has C. albicans Gcn4p (CaGcn4p) and CaGal4p with DNA binding domains highly similar to their S. cerevisiae counterparts. Deletion analysis of the CaGcn4p protein shows that the N' terminus is needed for transcriptional activation; an 81-amino-acid region is critical for this function, and this domain can be coupled to a lexA DNA binding module to provide transcription-activating function in a heterologous reporter system. Deletion analysis of the C. albicans Gal4p identifies a C-terminal 73-amino-acid-long transcription-activating domain that also can be transferred to a heterologous reporter construct to direct transcriptional activation. These two transcriptional activation regions show no sequence similarity to the respective domains in their S. cerevisiae homologs, and the two C. albicans transcription-activating domains themselves show little similarity.

Published ahead of print on 8 December 2006.

Supplemental material for this article may be found at http://ec.asm.org/.

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