Eukaryotic Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
EC.00204-06v1
6/2/245    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Strmecki, L.
Right arrow Articles by Pears, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Strmecki, L.
Right arrow Articles by Pears, C.

 Previous Article  |  Next Article 

Eukaryotic Cell, February 2007, p. 245-252, Vol. 6, No. 2
1535-9778/07/$08.00+0     doi:10.1128/EC.00204-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Proteomic and Microarray Analyses of the Dictyostelium Zak1-GSK-3 Signaling Pathway Reveal a Role in Early Development{triangledown}

Lana Strmecki,1 Gareth Bloomfield,2 Tsuyoshi Araki,3 Emma Dalton,4 Jason Skelton,5 Christina Schilde,3,{dagger} Adrian Harwood,4 Jeffrey G. Williams,3 Al Ivens,5 and Catherine Pears1*

Biochemistry Department, Oxford University, South Parks Rd., Oxford OX1 3QU, United Kingdom,1 MRC Laboratory of Molecular Biology, Hills Rd., Cambridge CB2 2QH, United Kingdom,2 School of Life Sciences, Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EJ, United Kingdom,3 Cardiff School of Biosciences, Cardiff University, Museum Ave., Cardiff CF10 3US, United Kingdom,4 The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, United Kingdom5

Received 28 June 2006/ Accepted 26 October 2006

GskA, the Dictyostelium GSK-3 orthologue, is modified and activated by the dual-specificity tyrosine kinase Zak1, and the two kinases form part of a signaling pathway that responds to extracellular cyclic AMP. We identify potential cellular effectors for the two kinases by analyzing the corresponding null mutants. There are proteins and mRNAs that are altered in abundance in only one or the other of the two mutants, indicating that each kinase has some unique functions. However, proteomic and microarray analyses identified a number of proteins and genes, respectively, that are similarly misregulated in both mutant strains. The positive correlation between the array data and the proteomic data is consistent with the Zak1-GskA signaling pathway's functioning by directly or indirectly regulating gene expression. The discoidin 1 genes are positively regulated by the pathway, while the abundance of the H5 protein is negatively regulated. Two of the targets, H5 and discoidin 1, are well-characterized markers for early development, indicating that the Zak1-GskA pathway plays a role in development earlier than previously observed.

* Corresponding author. Mailing address: Biochemistry Dept., Oxford University, South Parks Rd., Oxford OX1 3QU, United Kingdom. Phone: 44 1865 275737. Fax: 44 1865 275259. E-mail: pears{at}bioch.ox.ac.uk.

{triangledown} Published ahead of print on 3 November 2006.

{dagger} Present address: Universität Konstanz, FB Biologie, Universitätsstrasse 10, 78457 Konstanz, Germany.

Eukaryotic Cell, February 2007, p. 245-252, Vol. 6, No. 2
1535-9778/07/$08.00+0     doi:10.1128/EC.00204-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Appl. Environ. Microbiol. Infect. Immun. J. Bacteriol.
Mol. Cell Biol. Microbiol. Mol. Biol. Rev. ALL ASM JOURNALS
Copyright © 2007 by the American Society for Microbiology.