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Functional Characterization of Pneumocystis carinii brl1 by Transspecies Complementation Analysis ^,

Libera Lo Presti,^1, Moira Cockell,^1, Lorenzo Cerutti,^2, Viesturs Simanis,³ and Philippe M. Hauser^1*

Institute of Microbiology, Centre Hospitalier Universitaire Vaudois and University of Lausanne, Lausanne, Switzerland,¹ Swiss Institute of Bioinformatics, Lausanne, Switzerland,² Cell Cycle Control Laboratory, Swiss Institute for Experimental Cancer Research (ISREC), and School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne (EPFL), Epalinges, Switzerland³

Received 28 August 2007/ Accepted 19 October 2007

Pneumocystis jirovecii is a fungus which causes severe opportunistic infections in immunocompromised humans. The brl1 gene of P. carinii infecting rats was identified and characterized by using bioinformatics in conjunction with functional complementation in Saccharomyces cerevisiae and Schizosaccharomyces pombe. The ectopic expression of this gene rescues null alleles of essential nuclear membrane proteins of the Brr6/Brl1 family in both yeasts.

Published ahead of print on 9 November 2007.

Supplemental material for this article may be found at http://ec.asm.org/.

These authors contributed approximately equally to this work.

Present address: Ludwig Institute for Cancer Research, Lausanne, Switzerland.