Eukaryotic Cell MMBR Online 2003
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Eukaryotic Cell, December 2007, p. 2406-2418, Vol. 6, No. 12
1535-9778/07/$08.00+0     doi:10.1128/EC.00222-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Endocytosis in the Shiitake Mushroom Lentinula edodes and Involvement of GTPase LeRAB7{triangledown}

Ming Tsung Lee,1 Carol Ying Ying Szeto,2 Tak Pan Ng,1 and Hoi Shan Kwan1,2*

Department of Biology,1 and Molecular Biotechnology Program, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, SAR, People's Republic of China2

Received 25 June 2007/ Accepted 21 September 2007

Endocytosis is the process by which substrates enter a cell without passing through the plasma membrane but rather invaginate the cell membrane and form intracellular vesicles. Rab7 regulates endocytic trafficking between early and late endosomes and between late endosomes and lysosomes. LeRab7 in Lentinula edodes is strongly homologous to Rab7 in Homo sapiens. Receptors for activated C kinase-1 (LeRACK1) and Rab5 GTPase (LeRAB5) were isolated as interacting partners of LeRab7, and the interactions were confirmed by in vivo and in vitro protein interaction assays. The three genes showed differential expression in the various developmental stages of the mushroom. In situ hybridization showed that the three transcripts were localized in regions of active growth, such as the outer region of trama cells, and the subhymenium of the hymenophore of mature fruiting bodies and the prehymenophore of young fruiting bodies. The existence of endocytosis in the mycelium and hymenophores was confirmed by the internalization of FM4-64. LeRAB7 was partially colocalized with the AM4-64 and was located in the late endocytic pathway. This is the first report of the presence of endocytosis in homobasidiomycetes. LeRAB7, LeRAB5, and LeRACK1 may contribute to the growth of L. edodes and cell differentiation in hymenophores.

* Corresponding author. Mailing address: Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, SAR, People's Republic of China. Phone: (852) 2609-6251. Fax: (852) 2603-5315. E-mail: hoishankwan{at}cuhk.edu.hk

{triangledown} Published ahead of print on 5 October 2007.

Eukaryotic Cell, December 2007, p. 2406-2418, Vol. 6, No. 12
1535-9778/07/$08.00+0     doi:10.1128/EC.00222-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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