Eukaryotic Cell
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Eukaryotic Cell, November 2007, p. 2163-2167, Vol. 6, No. 11
1535-9778/07/$08.00+0     doi:10.1128/EC.00324-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Two Iron-Responsive Promoter Elements Control Expression of FOX1 in Chlamydomonas reinhardtii{triangledown}

Xiaodong Deng{dagger} and Mats Eriksson*

Umeå Plant Science Center, Umeå University, SE-901 87 Umeå, Sweden

Received 30 August 2007/ Accepted 11 September 2007

FOX1 encodes an iron deficiency-induced ferroxidase involved in a high-affinity iron uptake system. Mutagenesis analysis of the FOX1 promoter identified two separate iron-responsive elements, FeRE1 (CACACG) and FeRE2 (CACGCG), between positions –87 and –82 and between positions –65 and –60, respectively, and both are needed for induced FOX1 expression under conditions of iron deficiency.


* Corresponding author. Mailing address: Umeå Plant Science Center, Umeå University, SE-901 87 Umeå, Sweden. Phone: 46-90-7866918. Fax: 46-90-7866676. E-mail: mats.eriksson{at}plantphys.umu.se

{triangledown} Published ahead of print on 28 September 2007.

{dagger} Present address: State Key Laboratory of Tropical Crop Biotechnology, Institute of Tropical Bioscience and Biotechnology, Chinese Agricultural Academy for Tropical Crops, Haikou 571101, China.


Eukaryotic Cell, November 2007, p. 2163-2167, Vol. 6, No. 11
1535-9778/07/$08.00+0     doi:10.1128/EC.00324-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.




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