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Eukaryotic Cell, November 2007, p. 2157-2162, Vol. 6, No. 11
1535-9778/07/$08.00+0     doi:10.1128/EC.00304-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Sexual Pheromone Induces Diffusion of the Pheromone-Homologous Polypeptide in the Extracellular Matrix of Volvox carteri{triangledown}

Koichi Ishida*

Lehrstuhl Biochemie I, Universität Regensburg, D-93040 Regensburg, Germany

Received 17 August 2007/ Accepted 14 September 2007

The C-terminal domain of pherophorin II is homologous to the sexual pheromone of Volvox carteri and is released from other domains during sexual induction. Green fluorescent protein fused to the C terminus of pherophorin II was located at the extracellular matrix directly surrounding the gonidium, the final target of the sexual-induction signal.

* Present address: Nishii Initiative Research Unit, Frontier Research System, RIKEN, Hirosawa 2-1, Wako, 351-0198 Saitama, Japan. Phone: 81-48-467-4834. Fax: 81-48-467-9530. E-mail: koichi-ishida{at}riken.jp

{triangledown} Published ahead of print on 28 September 2007.

Eukaryotic Cell, November 2007, p. 2157-2162, Vol. 6, No. 11
1535-9778/07/$08.00+0     doi:10.1128/EC.00304-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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