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Eukaryotic Cell, November 2007, p. 1992-2000, Vol. 6, No. 11
1535-9778/07/$08.00+0     doi:10.1128/EC.00197-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

An Sfi1p-Like Centrin-Binding Protein Mediates Centrin-Based Ca2+-Dependent Contractility in Paramecium tetraurelia{triangledown} ,{dagger}

Delphine Gogendeau,1,2,3 Janine Beisson,1,2,3 Nicole Garreau de Loubresse,1,2,3 Jean-Pierre Le Caer,4 Françoise Ruiz,1,2,3 Jean Cohen,1,2,3 Linda Sperling,1,2,3 France Koll,1,2,3 and Catherine Klotz1,2,3*

Centre de Génétique Moléculaire, UPR 2167,1 Institut de Chimie des Substances Naturelles, UPR 2301, CNRS, F-91198 Gif-sur-Yvette,4 Université Paris-Sud, F-91405 Orsay,2 Université Pierre et Marie Curie, Paris 6, F-75005 Paris, France3

Received 4 June 2007/ Accepted 19 July 2007

The previous characterization and structural analyses of Sfi1p, a Saccharomyces cerevisiae centrin-binding protein essential for spindle pole body duplication, have suggested molecular models to account for centrin-mediated, Ca2+-dependent contractility processes (S. Li, A. M. Sandercock, P. Conduit, C. V. Robinson, R. L. Williams, and J. V. Kilmartin, J. Cell Biol. 173:867-877, 2006). Such processes can be analyzed by using Paramecium tetraurelia, which harbors a large Ca2+-dependent contractile cytoskeletal network, the infraciliary lattice (ICL). Previous biochemical and genetic studies have shown that the ICL is composed of diverse centrin isoforms and a high-molecular-mass centrin-associated protein, whose reduced size in the démaillé (dem1) mutant correlates with defective organization of the ICL. Using sequences derived from the high-molecular-mass protein to probe the Paramecium genome sequence, we characterized the PtCenBP1 gene, which encodes a 460-kDa protein. PtCenBP1p displays six almost perfect repeats of ca. 427 amino acids (aa) and harbors 89 potential centrin-binding sites with the consensus motif LLX11F/LX2WK/R, similar to the centrin-binding sites of ScSfi1p. The smaller (260-kDa) protein encoded by the dem1 mutant PtCenBP1 allele comprises only two repeats of 427 aa and 46 centrin-binding sites. By using RNA interference and green fluorescent protein fusion experiments, we showed that PtCenBP1p forms the backbone of the ICL and plays an essential role in its assembly and contractility. This study provides the first in vivo demonstration of the role of Sfi1p-like proteins in centrin-mediated Ca2+-dependent contractile processes.

* Corresponding author. Mailing address: Centre de Génétique Moléculaire, UPR 2167, Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette, France. Phone: 33 1 6982 4392. Fax: 33 1 6982 3181. E-mail: klotz{at}cgm.cnrs-gif.fr

{triangledown} Published ahead of print on 3 August 2007.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

Eukaryotic Cell, November 2007, p. 1992-2000, Vol. 6, No. 11
1535-9778/07/$08.00+0     doi:10.1128/EC.00197-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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