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Eukaryotic Cell, October 2007, p. 1913-1924, Vol. 6, No. 10
1535-9778/07/$08.00+0     doi:10.1128/EC.00217-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Class I Histone Deacetylase Thd1p Promotes Global Chromatin Condensation in Tetrahymena thermophila{triangledown}

Kathryn Parker,2 Julia Maxson,1 Alissa Mooney,1 and Emily A. Wiley1*

Joint Science Department, W. M. Keck Science Center, Claremont Colleges, Claremont, California 91711,1 Department of Biology, Mount Holyoke College, South Hadley, Massachusetts 010752

Received 22 June 2007/ Accepted 8 August 2007

Class I histone deacetylases (HDACs) regulate DNA-templated processes such as transcription. They act both at specific loci and more generally across global chromatin, contributing to acetylation patterns that may underlie large-scale chromatin dynamics. Although hypoacetylation is correlated with highly condensed chromatin, little is known about the contribution of individual HDACs to chromatin condensation mechanisms. Using the ciliated protozoan Tetrahymena thermophila, we investigated the role of a specific class I HDAC, Thd1p, in the reversible condensation of global chromatin. In this system, the normal physiological response to cell starvation includes the widespread condensation of the macronuclear chromatin and general repression of gene transcription. We show that the chromatin in Thd1p-deficient cells failed to condense during starvation. The condensation failure correlated with aberrant hyperphosphorylation of histone H1 and the overexpression of CDC2, encoding the major histone H1 kinase. Changes in the rate of acetate turnover on core histones and in the distribution of acetylated lysines 9 and 23/27 on histone H3 isoforms that were found to correlate with normal chromatin condensation were absent from Thd1p mutant cells. These results point to a role for a class I HDAC in the formation of reversible higher-order chromatin structures and global genome compaction through mechanisms involving the regulation of H1 phosphorylation and core histone acetylation/deacetylation kinetics.

* Corresponding author. Mailing address: Joint Science Department, W. M. Keck Science Center, 925 N. Mills Ave., Claremont, CA 91711. Phone: (909) 607-9698. Fax: (909) 621-8555. E-mail: ewiley{at}jsd.claremont.edu

{triangledown} Published ahead of print on 22 August 2007.

Eukaryotic Cell, October 2007, p. 1913-1924, Vol. 6, No. 10
1535-9778/07/$08.00+0     doi:10.1128/EC.00217-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Smith, J. J., Torigoe, S. E., Maxson, J., Fish, L. C., Wiley, E. A. (2008). A Class II Histone Deacetylase Acts on Newly Synthesized Histones in Tetrahymena. Eukaryot Cell 7: 471-482 [Abstract] [Full Text]  


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