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Eukaryotic Cell, October 2007, p. 1782-1794, Vol. 6, No. 10
1535-9778/07/$08.00+0     doi:10.1128/EC.00155-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Differential Involvement of Histidine Kinase Receptors in Pseudohyphal Development, Stress Adaptation, and Drug Sensitivity of the Opportunistic Yeast Candida lusitaniae{triangledown}

Florence Chapeland-Leclerc,* Paméla Paccallet, Gwenaël Ruprich-Robert, David Reboutier, Christiane Chastin, and Nicolas Papon

Programme Chimiorésistance des Levures Pathogènes, EA209 Eucaryotes Pathogènes: Transports Membranaires et Chimiorésistance, UFR des Sciences Pharmaceutiques et Biologiques, Université Paris-Descartes, 75006 Paris, France

Received 2 May 2007/ Accepted 19 July 2007

Fungal histidine kinase receptors (HKRs) sense and transduce many extracellular signals. We investigated the role of HKRs in morphogenetic transition, osmotolerance, oxidative stress response, and mating ability in the opportunistic yeast Candida lusitaniae. We isolated three genes, SLN1, NIK1, and CHK1, potentially encoding HKRs of classes VI, III, and X, respectively. These genes were disrupted by a transformation system based upon the "URA3 blaster" strategy. Functional analysis of disruptants was undertaken, except for the sln1 nik1 double mutant and the sln1 nik1 chk1 triple mutant, which are not viable in C. lusitaniae. The sln1 mutant revealed a high sensitivity to oxidative stress, whereas both the nik1 and chk1 mutants exhibited a more moderate sensitivity to peroxide. We also showed that the NIK1 gene was implicated in phenylpyrrole and dicarboximide compound susceptibility while HKRs seem not to be involved in resistance toward antifungals of clinical relevance. Concerning mating ability, all disruptants were still able to reproduce sexually in vitro in unilateral or bilateral crosses. The most important result of this study was that the sln1 mutant displayed a global defect of pseudohyphal differentiation, especially in high-osmolarity and oxidative-stress conditions. Thus, the SLN1 gene could be crucial for the C. lusitaniae yeast-to-pseudohypha morphogenetic transition. This implication is strengthened by a high level of SLN1 mRNAs revealed by semiquantitative reverse transcription-PCR when the yeast develops pseudohyphae. Our findings highlight a differential contribution of the three HKRs in osmotic and oxidant adaptation during the morphological transition in C. lusitaniae.

* Corresponding author. Mailing address: Programme Chimiorésistance des Levures Pathogènes, EA209 Eucaryotes Pathogènes: Transports Membranaires et Chimiorésistance, UFR des Sciences Pharmaceutiques et Biologiques, Université Paris-Descartes, 4 avenue de l'Observatoire, 75006 Paris, France. Phone: (33) 1 53 73 96 42. Fax: (33) 1 53 73 96 40. E-mail: florence.leclerc{at}univ-paris5.fr

{triangledown} Published ahead of print on 27 July 2007.

Eukaryotic Cell, October 2007, p. 1782-1794, Vol. 6, No. 10
1535-9778/07/$08.00+0     doi:10.1128/EC.00155-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Florent, M., Noel, T., Ruprich-Robert, G., Da Silva, B., Fitton-Ouhabi, V., Chastin, C., Papon, N., Chapeland-Leclerc, F. (2009). Nonsense and Missense Mutations in FCY2 and FCY1 Genes Are Responsible for Flucytosine Resistance and Flucytosine-Fluconazole Cross-Resistance in Clinical Isolates of Candida lusitaniae. Antimicrob. Agents Chemother. 53: 2982-2990 [Abstract] [Full Text]  
  • Boisnard, S., Ruprich-Robert, G., Florent, M., Da Silva, B., Chapeland-Leclerc, F., Papon, N. (2008). Insight into the Role of HOG Pathway Components Ssk2p, Pbs2p, and Hog1p in the Opportunistic Yeast Candida lusitaniae. Eukaryot Cell 7: 2179-2183 [Abstract] [Full Text]  
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  • Ruprich-Robert, G., Chapeland-Leclerc, F., Boisnard, S., Florent, M., Bories, G., Papon, N. (2008). Contributions of the Response Regulators Ssk1p and Skn7p in the Pseudohyphal Development, Stress Adaptation, and Drug Sensitivity of the Opportunistic Yeast Candida lusitaniae. Eukaryot Cell 7: 1071-1074 [Abstract] [Full Text]  


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