This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fleißner, A.
Right arrow Articles by Glass, N. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fleißner, A.
Right arrow Articles by Glass, N. L.

 Previous Article  |  Next Article 

Eukaryotic Cell, January 2007, p. 84-94, Vol. 6, No. 1
1535-9778/07/$08.00+0     doi:10.1128/EC.00268-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SO, a Protein Involved in Hyphal Fusion in Neurospora crassa, Localizes to Septal Plugs{triangledown} ,{dagger}

André Fleißner and N. Louise Glass*

Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, California 94720

Received 22 August 2006/ Accepted 31 October 2006

The colony of a filamentous ascomycete fungus typically grows as a multinucleate syncytium. While this syncytial organization has developmental advantages, it bears the risk of extensive damage caused by local injury of hyphae. Loss of cytoplasm in injured hyphae is restricted by the fast and efficient sealing of the central pores of hyphal crosswalls, or septa, by a peroxisome-derived organelle called the Woronin body. The formation of septal plugs is also associated with development and leads to separation of certain parts of the colony. Septal plugs associated with developmental processes or aging hyphae typically occur by the accumulation of sealing material. Here we report that in Neurospora crassa, a protein necessary for hyphal fusion and proper colony development called SO (SOFT) localizes to septal plugs. In response to injury, SO accumulates at the septal plug in a Woronin body-independent manner. However, the presence of the Woronin body affects the speed of accumulation of SO at the septal pore. We determined that SO contributes to, but is not essential for, septal plugging. SO accumulation was also observed at septal plugs formed during hyphal aging and during programmed cell death mediated by genetic differences at heterokaryon incompatibility (het) loci.

* Corresponding author. Mailing address: Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720-3102. Phone: (510) 643-2399. Fax: (510) 642-4995. E-mail: Lglass{at}nature.berkeley.edu.

{triangledown} Published ahead of print on 10 November 2006.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

Eukaryotic Cell, January 2007, p. 84-94, Vol. 6, No. 1
1535-9778/07/$08.00+0     doi:10.1128/EC.00268-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Craven, K. D., Velez, H., Cho, Y., Lawrence, C. B., Mitchell, T. K. (2008). Anastomosis Is Required for Virulence of the Fungal Necrotroph Alternaria brassicicola. Eukaryot Cell 7: 675-683 [Abstract] [Full Text]  
  • Prados Rosales, R. C., Di Pietro, A. (2008). Vegetative Hyphal Fusion Is Not Essential for Plant Infection by Fusarium oxysporum. Eukaryot Cell 7: 162-171 [Abstract] [Full Text]  
  • Engh, I., Wurtz, C., Witzel-Schlomp, K., Zhang, H. Y., Hoff, B., Nowrousian, M., Rottensteiner, H., Kuck, U. (2007). The WW Domain Protein PRO40 Is Required for Fungal Fertility and Associates with Woronin Bodies. Eukaryot Cell 6: 831-843 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»