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Eukaryotic Cell, January 2007, p. 110-116, Vol. 6, No. 1
1535-9778/07/$08.00+0     doi:10.1128/EC.00233-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Molecular Systematics of Dictyostelids: 5.8S Ribosomal DNA and Internal Transcribed Spacer Region Analyses{triangledown}

María Romeralo,1 Ricardo Escalante,2 Leandro Sastre,2* and Carlos Lado1

Real Jardín Botánico CSIC, Plaza de Murillo, 2, 28014 Madrid, Spain,1 Instituto de Investigaciones Biomédicas CSIC/UAM, Arturo Duperier, 4, 28029 Madrid, Spain2

Received 18 July 2006/ Accepted 26 September 2006

The variability and adaptability of the amoebae from the class Dictyosteliomycetes greatly complicate their systematics. The nucleotide sequences of the ribosomal internal transcribed spacers and the 5.8S ribosomal DNA gene have been determined for 28 isolates, and their utility to discriminate between different species and genera has been shown.


* Corresponding author. Mailing address: Instituto de Investigaciones Biomédicas, Arturo Duperier, 4, 28029 Madrid, Spain. Phone: 34 915854437. Fax: 34 915854401. E-mail: lsastre{at}iib.uam.es.

{triangledown} Published ahead of print on 20 October 2006.


Eukaryotic Cell, January 2007, p. 110-116, Vol. 6, No. 1
1535-9778/07/$08.00+0     doi:10.1128/EC.00233-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.




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