This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hernandez-Lopez, M. J.
Right arrow Articles by Prieto, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hernandez-Lopez, M. J.
Right arrow Articles by Prieto, J. A.

 Previous Article  |  Next Article 

Eukaryotic Cell, August 2006, p. 1410-1419, Vol. 5, No. 8
1535-9778/06/$08.00+0     doi:10.1128/EC.00068-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Hog1 Mitogen-Activated Protein Kinase Plays Conserved and Distinct Roles in the Osmotolerant Yeast Torulaspora delbrueckii{dagger}

María José Hernandez-Lopez, Francisca Randez-Gil, and José Antonio Prieto*

Department of Biotechnology, Instituto de Agroquímica y Tecnología de los Alimentos, Consejo Superior de Investigaciones Científicas, P.O. Box 73, E-46100 Burjassot, Valencia, Spain

Received 7 March 2006/ Accepted 5 June 2006

Torulaspora delbrueckii has emerged during evolution as one of the most osmotolerant yeasts. However, the molecular mechanisms underlying this unusual stress resistance are poorly understood. In this study, we have characterized the functional role of the high-osmolarity glycerol (HOG) mitogen-activated protein kinase pathway in mediating the osmotic stress response, among others, in T. delbrueckii. We show that the T. delbrueckii Hog1p homologue TdHog1p is phosphorylated after cell transfer to NaCl- or sorbitol-containing medium. However, TdHog1p plays a minor role in tolerance to conditions of moderate osmotic stress, a trait related mainly with the osmotic balance. In consonance with this, the absence of TdHog1p produced only a weak defect in the timing of the osmostress-induced glycerol and GPD1 mRNA overaccumulation. Tdhog1{Delta} mutants also failed to display aberrant morphology changes in response to osmotic stress. Furthermore, our data indicate that the T. delbrueckii HOG pathway has evolved to respond to specific environmental conditions and to play a pivotal role in the stress cross-protection mechanism.

* Corresponding author. Mailing address: Department of Biotechnology, Instituto de Agroquímica y Tecnología de los Alimentos, Consejo Superior de Investigaciones Científicas, P.O. Box 73, E-46100 Burjassot, Valencia, Spain. Phone: 34-963900022. Fax: 34-963636301. E-mail: prieto{at}iata.csic.es.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

Eukaryotic Cell, August 2006, p. 1410-1419, Vol. 5, No. 8
1535-9778/06/$08.00+0     doi:10.1128/EC.00068-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Boisnard, S., Ruprich-Robert, G., Florent, M., Da Silva, B., Chapeland-Leclerc, F., Papon, N. (2008). Insight into the Role of HOG Pathway Components Ssk2p, Pbs2p, and Hog1p in the Opportunistic Yeast Candida lusitaniae. Eukaryot Cell 7: 2179-2183 [Abstract] [Full Text]  
  • Panadero, J., Hernandez-Lopez, M. J., Prieto, J. A., Randez-Gil, F. (2007). Overexpression of the Calcineurin Target CRZ1 Provides Freeze Tolerance and Enhances the Fermentative Capacity of Baker's Yeast. Appl. Environ. Microbiol. 73: 4824-4831 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»