This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Johnsson, A.
Right arrow Articles by Wright, A. P. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Johnsson, A.
Right arrow Articles by Wright, A. P. H.

 Previous Article  |  Next Article 

Eukaryotic Cell, August 2006, p. 1337-1346, Vol. 5, No. 8
1535-9778/06/$08.00+0     doi:10.1128/EC.00101-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Stress-Specific Role of Fission Yeast Gcn5 Histone Acetyltransferase in Programming a Subset of Stress Response Genes{dagger}

Anna Johnsson,* Yongtao Xue-Franzén, Maria Lundin, and Anthony P. H. Wright

School of Life Sciences, Södertörns Högskola, and Department of Biosciences and Nutrition, Karolinska Institute, SE-141 89 Huddinge, Sweden

Received 12 April 2006/ Accepted 18 June 2006

Gcn5 is a coactivator protein that contributes to gene activation by acetylating specific lysine residues within the N termini of histone proteins. Gcn5 has been intensively studied in the budding yeast, Saccharomyces cerevisiae, but the features of genes that determine whether they require Gcn5 during activation have not been conclusively clarified. To allow comparison with S. cerevisiae, we have studied the genome-wide role of Gcn5 in the distantly related fission yeast, Schizosaccharomyces pombe. We show that Gcn5 is specifically required for adaptation to KCl- and CaCl2-mediated stress in S. pombe. We have characterized the genome-wide gene expression responses to KCl stress and show that Gcn5 is involved in the regulation of a subset of stress response genes. Gcn5 is most clearly associated with KCl-induced genes, but there is no correlation between Gcn5 dependence and the extent of their induction. Instead, Gcn5-dependent KCl-induced genes are specifically enriched in four different DNA motifs. The Gcn5-dependent KCl-induced genes are also associated with biological process gene ontology terms such as carbohydrate metabolism, glycolysis, and nicotinamide metabolism that together constitute a subset of the ontology parameters associated with KCl-induced genes.

* Corresponding author. Mailing address: School of Life Sciences, Södertörns Högskola, SE-141 89 Huddinge, Sweden. Phone: 46-8-6084709. Fax: 46-8-6084510. E-mail: anna.johnsson{at}sh.se.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

Eukaryotic Cell, August 2006, p. 1337-1346, Vol. 5, No. 8
1535-9778/06/$08.00+0     doi:10.1128/EC.00101-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Wellen, K. E., Hatzivassiliou, G., Sachdeva, U. M., Bui, T. V., Cross, J. R., Thompson, C. B. (2009). ATP-Citrate Lyase Links Cellular Metabolism to Histone Acetylation. Science 324: 1076-1080 [Abstract] [Full Text]  
  • Helmlinger, D., Marguerat, S., Villen, J., Gygi, S. P., Bahler, J., Winston, F. (2008). The S. pombe SAGA complex controls the switch from proliferation to sexual differentiation through the opposing roles of its subunits Gcn5 and Spt8. Genes Dev. 22: 3184-3195 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»