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Eukaryotic Cell, July 2006, p. 1043-1056, Vol. 5, No. 7
1535-9778/06/$08.00+0     doi:10.1128/EC.00086-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

A MADS Box Protein Interacts with a Mating-Type Protein and Is Required for Fruiting Body Development in the Homothallic Ascomycete Sordaria macrospora

Nicole Nolting and Stefanie Pöggeler*

Department of General and Molecular Botany, Ruhr University of Bochum, 44780 Bochum, Germany

Received 23 March 2006/ Accepted 1 May 2006

MADS box transcription factors control diverse developmental processes in plants, metazoans, and fungi. To analyze the involvement of MADS box proteins in fruiting body development of filamentous ascomycetes, we isolated the mcm1 gene from the homothallic ascomycete Sordaria macrospora, which encodes a putative homologue of the Saccharomyces cerevisiae MADS box protein Mcm1p. Deletion of the S. macrospora mcm1 gene resulted in reduced biomass, increased hyphal branching, and reduced hyphal compartment length during vegetative growth. Furthermore, the S. macrospora {Delta}mcm1 strain was unable to produce fruiting bodies or ascospores during sexual development. A yeast two-hybrid analysis in conjugation with in vitro analyses demonstrated that the S. macrospora MCM1 protein can interact with the putative transcription factor SMTA-1, encoded by the S. macrospora mating-type locus. These results suggest that the S. macrospora MCM1 protein is involved in the transcriptional regulation of mating-type-specific genes as well as in fruiting body development.

* Corresponding author. Mailing address: Department of General and Molecular Botany, Ruhr University of Bochum, ND6/161, Universitätsstrasse 150, 44780 Bochum, Germany. Phone: 49-234-3224264. Fax: 49-234-3214184. E-mail: Stefanie.Poeggeler{at}rub.de.

Eukaryotic Cell, July 2006, p. 1043-1056, Vol. 5, No. 7
1535-9778/06/$08.00+0     doi:10.1128/EC.00086-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Poggeler, S., Hoff, B., Kuck, U. (2008). Asexual Cephalosporin C Producer Acremonium chrysogenum Carries a Functional Mating Type Locus. Appl. Environ. Microbiol. 74: 6006-6016 [Abstract] [Full Text]  
  • Mehrabi, R., Ding, S., Xu, J.-R. (2008). MADS-Box Transcription Factor Mig1 Is Required for Infectious Growth in Magnaporthe grisea. Eukaryot Cell 7: 791-799 [Abstract] [Full Text]  


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