This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grossmann, G.
Right arrow Articles by Tanner, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grossmann, G.
Right arrow Articles by Tanner, W.

 Previous Article  |  Next Article 

Eukaryotic Cell, June 2006, p. 945-953, Vol. 5, No. 6
1535-9778/06/$08.00+0     doi:10.1128/EC.00206-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Lipid Raft-Based Membrane Compartmentation of a Plant Transport Protein Expressed in Saccharomyces cerevisiae

Guido Grossmann,1 Miroslava Opekarova,2 Linda Novakova,1 Jürgen Stolz,1 and Widmar Tanner1*

Institute of Cell Biology and Plant Physiology, University of Regensburg, 93040 Regensburg, Germany,1 Institute of Microbiology, CAS, 14220 Prague 4, Czech Republic2

Received 20 July 2005/ Accepted 4 November 2005

The hexose-proton symporter HUP1 shows a spotty distribution in the plasma membrane of the green alga Chlorella kessleri. Chlorella cannot be transformed so far. To study the membrane localization of the HUP1 protein in detail, the symporter was fused to green fluorescent protein (GFP) and heterologously expressed in Saccharomyces cerevisiae and Schizosaccharomyces pombe. In these organisms, the HUP1 protein has previously been shown to be fully active. The GFP fusion protein was exclusively targeted to the plasma membranes of both types of fungal cells. In S. cerevisiae, it was distributed nonhomogenously and concentrated in spots resembling the patchy appearance observed previously for endogenous H+ symporters. It is documented that the Chlorella protein colocalizes with yeast proteins that are concentrated in 300-nm raft-based membrane compartments. On the other hand, it is completely excluded from the raft compartment housing the yeast H+/ATPase. As judged by their solubilities in Triton X-100, the HUP1 protein extracted from Chlorella and the GFP fusion protein extracted from S. cerevisiae are detergent-resistant raft proteins. S. cerevisiae mutants lacking the typical raft lipids ergosterol and sphingolipids showed a homogenous distribution of HUP1-GFP within the plasma membrane. In an ergosterol synthesis (erg6) mutant, the rate of glucose uptake was reduced to less than one-third that of corresponding wild-type cells. In S. pombe, the sterol-rich plasma membrane domains can be stained in vivo with filipin. Chlorella HUP1-GFP accumulated exactly in these domains. Altogether, it is demonstrated here that a plant membrane protein has the property of being concentrated in specific raft-based membrane compartments and that the information for its raft association is retained between even distantly related organisms.

* Corresponding author. Mailing address: Institute of Cell Biology and Plant Physiology, University of Regensburg, 93040 Regensburg, Germany. Phone: 49 941 943-3018. Fax: 49 941 943-3352. E-mail: sekretariat.tanner{at}biologie.uni-regensburg.de.

Eukaryotic Cell, June 2006, p. 945-953, Vol. 5, No. 6
1535-9778/06/$08.00+0     doi:10.1128/EC.00206-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Stradalova, V., Stahlschmidt, W., Grossmann, G., Blazikova, M., Rachel, R., Tanner, W., Malinsky, J. (2009). Furrow-like invaginations of the yeast plasma membrane correspond to membrane compartment of Can1. J. Cell Sci. 122: 2887-2894 [Abstract] [Full Text]  
  • Raffaele, S., Bayer, E., Lafarge, D., Cluzet, S., German Retana, S., Boubekeur, T., Leborgne-Castel, N., Carde, J.-P., Lherminier, J., Noirot, E., Satiat-Jeunemaitre, B., Laroche-Traineau, J., Moreau, P., Ott, T., Maule, A. J., Reymond, P., Simon-Plas, F., Farmer, E. E., Bessoule, J.-J., Mongrand, S. (2009). Remorin, a Solanaceae Protein Resident in Membrane Rafts and Plasmodesmata, Impairs Potato virus X Movement. Plant Cell 21: 1541-1555 [Abstract] [Full Text]  
  • Berchtold, D., Walther, T. C. (2009). TORC2 Plasma Membrane Localization Is Essential for Cell Viability and Restricted to a Distinct Domain. Mol. Biol. Cell 20: 1565-1575 [Abstract] [Full Text]  
  • Grossmann, G., Malinsky, J., Stahlschmidt, W., Loibl, M., Weig-Meckl, I., Frommer, W. B., Opekarova, M., Tanner, W. (2008). Plasma membrane microdomains regulate turnover of transport proteins in yeast. JCB 183: 1075-1088 [Abstract] [Full Text]  
  • Krugel, U., Veenhoff, L. M., Langbein, J., Wiederhold, E., Liesche, J., Friedrich, T., Grimm, B., Martinoia, E., Poolman, B., Kuhn, C. (2008). Transport and Sorting of the Solanum tuberosum Sucrose Transporter SUT1 Is Affected by Posttranslational Modification. Plant Cell 20: 2497-2513 [Abstract] [Full Text]  
  • Vogl, C., Klein, C. M., Batke, A. F., Schweingruber, M. E., Stolz, J. (2008). Characterization of Thi9, a Novel Thiamine (Vitamin B1) Transporter from Schizosaccharomyces pombe. J. Biol. Chem. 283: 7379-7389 [Abstract] [Full Text]  
  • Iwaki, T., Iefuji, H., Hiraga, Y., Hosomi, A., Morita, T., Giga-Hama, Y., Takegawa, K. (2008). Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe. Microbiology 154: 830-841 [Abstract] [Full Text]  
  • Pasrija, R., Panwar, S. L., Prasad, R. (2008). Multidrug Transporters CaCdr1p and CaMdr1p of Candida albicans Display Different Lipid Specificities: both Ergosterol and Sphingolipids Are Essential for Targeting of CaCdr1p to Membrane Rafts. Antimicrob. Agents Chemother. 52: 694-704 [Abstract] [Full Text]  
  • Lauwers, E., Grossmann, G., Andre, B. (2007). Evidence for Coupled Biogenesis of Yeast Gap1 Permease and Sphingolipids: Essential Role in Transport Activity and Normal Control by Ubiquitination. Mol. Biol. Cell 18: 3068-3080 [Abstract] [Full Text]  
  • Steinberg, G. (2007). Hyphal Growth: a Tale of Motors, Lipids, and the Spitzenkorper. Eukaryot Cell 6: 351-360 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»