Eukaryotic Cell
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Eukaryotic Cell, May 2006, p. 871-875, Vol. 5, No. 5
1535-9778/06/$08.00+0     doi:10.1128/EC.5.5.871-875.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Stress by Heat Shock Induces Massive Down Regulation of Genes and Allows Differential Allelic Expression of the Gal/GalNAc Lectin in Entamoeba histolytica{dagger}

Christian Weber,1 Ghislaine Guigon,2 Christiane Bouchier,2 Lionel Frangeul,2 Sandrine Moreira,2 Odile Sismeiro,2 Catherine Gouyette,2 David Mirelman,3 Jean Yves Coppee,2 and Nancy Guillén1*

Unité Biologie Cellulaire du Parasitisme, INSERM 786, Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris Cedex 15, France,1 Pasteur Genopole Île-de-France, Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris Cedex 15, France,2 Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot, Israel3

Received 8 December 2005/ Accepted 21 February 2006

Gene expression analysis by microarray assay revealed that when exposed to stress, Entamoeba histolytica exhibits a specific heat shock response, together with a dramatic overall reduction in gene transcription as well as differential allelic expression of key genes participating in virulence, such as the galactose/N-acetylgalactosamine (Gal/GalNAc) lectin.

* Corresponding author. Mailing address: Unité Biologie Cellulaire du Parasitisme, INSERM 786, Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris Cedex 15, France. Phone: 331-45688675. Fax: 331-45688674. E-mail: nguillen{at}pasteur.fr.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

Eukaryotic Cell, May 2006, p. 871-875, Vol. 5, No. 5
1535-9778/06/$08.00+0     doi:10.1128/EC.5.5.871-875.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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