This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schubert, D.
Right arrow Articles by Kothe, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schubert, D.
Right arrow Articles by Kothe, E.

 Previous Article  |  Next Article 

Eukaryotic Cell, April 2006, p. 683-695, Vol. 5, No. 4
1535-9778/06/$08.00+0     doi:10.1128/EC.5.4.683-695.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Ras GTPase-Activating Protein Gap1 of the Homobasidiomycete Schizophyllum commune Regulates Hyphal Growth Orientation and Sexual Development

Daniela Schubert,1 Marjatta Raudaskoski,2 Nicole Knabe,1 and Erika Kothe1*

Institute of Microbiology, Microbial Phytopathology, Friedrich-Schiller-University, D-07749 Jena, Germany,1 Department of Biology, Fin-20014 University of Turku, Finland2

Received 26 September 2005/ Accepted 3 January 2006

The white rot fungus Schizophyllum commune is used for the analysis of mating and sexual development in homobasidiomycete fungi. In this study, we isolated the gene gap1 encoding a GTPase-activating protein for Ras. Disruption of gap1 should therefore lead to strains accumulating Ras in its activated, GTP-bound state and to constitutive Ras signaling. Haploid {Delta}gap1 monokaryons of different mating types did not show alterations in mating behavior in the four different mating interactions possible in fungi expressing a tetrapolar mating type system. Instead, the growth rate in {Delta}gap1 monokaryons was reduced by ca. 25% and ca. 50% in homozygous {Delta}gap1/{Delta}gap1 dikaryons. Monokaryons, as well as homozygous dikaryons, carrying the disrupted gap1 alleles exhibited a disorientated growth pattern. Dikaryons showed a strong phenotype during clamp formation since hook cells failed to fuse with the peg beside them. Instead, the dikaryotic character of the hyphae was rescued by fusion of the hooks with nearby developing branches. {Delta}gap1/{Delta}gap1 dikaryons formed increased numbers of fruitbody primordia, whereas the amount of fruitbodies was not raised. Mature fruitbodies formed no or abnormal gills. No production of spores could be observed. The results suggest Ras involvement in growth, clamp formation, and fruitbody development.

* Corresponding author. Mailing address: Institute of Microbiology, Microbial Phytopathology, Neugasse 25, D-07743 Jena, Germany. Phone: 49-3641-949291. Fax: 49-3641-949292. E-mail: Erika.Kothe{at}uni-jena.de.

Eukaryotic Cell, April 2006, p. 683-695, Vol. 5, No. 4
1535-9778/06/$08.00+0     doi:10.1128/EC.5.4.683-695.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Harispe, L., Portela, C., Scazzocchio, C., Penalva, M. A., Gorfinkiel, L. (2008). Ras GTPase-Activating Protein Regulation of Actin Cytoskeleton and Hyphal Polarity in Aspergillus nidulans. Eukaryot Cell 7: 141-153 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»