This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hernandez-Lopez, M. J.
Right arrow Articles by Randez-Gil, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hernandez-Lopez, M. J.
Right arrow Articles by Randez-Gil, F.

 Previous Article  |  Next Article 

Eukaryotic Cell, March 2006, p. 469-479, Vol. 5, No. 3
1535-9778/06/$08.00+0     doi:10.1128/EC.5.3.469-479.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Regulation of Salt Tolerance by Torulaspora delbrueckii Calcineurin Target Crz1p

Maria Jose Hernandez-Lopez, Joaquin Panadero, Jose Antonio Prieto, and Francisca Randez-Gil*

Department of Biotechnology, Instituto de Agroquímica y Tecnología de los Alimentos, Consejo Superior de Investigaciones Científicas, P.O. Box 73, E-46100 Burjassot, Valencia, Spain

Received 27 July 2005/ Accepted 28 November 2005

Recently, the academic interest in the yeast Torulaspora delbrueckii has increased notably due to its high resistance to several types of stress, including salt and osmotic imbalance. However, the molecular mechanisms underlying these unusual properties are poorly understood. In Saccharomyces cerevisiae, the high-salt response is mediated by calcineurin, a conserved Ca2+/calmodulin-modulated protein phosphatase that regulates the transcriptional factor Crz1p. Here, we cloned the T. delbrueckii TdCRZ1 gene, which encodes a putative zinc finger transcription factor homologue to Crz1p. Consistent with this, overexpression of TdCRZ1 enhanced the salt tolerance of S. cerevisiae wild-type cells and suppressed the sensitivity phenotype of cnb1{Delta} and crz1{Delta} mutants to monovalent and divalent cations. However, T. delbrueckii cells lacking TdCrz1p showed phenotypes distinct from those previously observed in S. cerevisiae crz1{Delta} mutants. Quite remarkably, Tdcrz1-null cells were insensitive to high Na+ and were more Li+ tolerant than wild-type cells. Clearly, TdCrz1p was not required for the salt-induced transcriptional activation of the TdENA1 gene, encoding a putative P-type ATPase homologue to the main S. cerevisiae Na+ pump ENA1. Furthermore, T. delbrueckii cells were insensitive to the immunosuppressive agents FK506 and cyclosporine A, both in the presence and in the absence of NaCl. Signaling through the calcineurin/Crz1 pathway appeared to be essential only on high-Ca2+/Mn2+ media. Hence, T. delbrueckii and S. cerevisiae differ in the regulatory circuits and mechanisms that drive the adaptive response to salt stress.

* Corresponding author. Mailing address: Department of Biotechnology, Instituto de Agroquímica y Tecnología de los Alimentos, Consejo Superior de Investigaciones Científicas, P.O. Box 73, E-46100 Burjassot, Valencia, Spain. Phone: 34-963900022. Fax: 34-963636301. E-mail: randez{at}iata.csic.es.

Eukaryotic Cell, March 2006, p. 469-479, Vol. 5, No. 3
1535-9778/06/$08.00+0     doi:10.1128/EC.5.3.469-479.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Schumacher, J., de Larrinoa, I. F., Tudzynski, B. (2008). Calcineurin-Responsive Zinc Finger Transcription Factor CRZ1 of Botrytis cinerea Is Required for Growth, Development, and Full Virulence on Bean Plants. Eukaryot Cell 7: 584-601 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»