Eukaryotic Cell
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Eukaryotic Cell, February 2006, p. 422-425, Vol. 5, No. 2
1535-9778/06/$08.00+0     doi:10.1128/EC.5.2.422-425.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Metallothionein Gene from Tetrahymena thermophila with a Copper-Inducible-Repressible Promoter

Francesco Boldrin,1* Gianfranco Santovito,1 Jacek Gaertig,2 Dorota Wloga,2 Donna Cassidy-Hanley,3 Theodore G. Clark,3 and Ester Piccinni1

Department of Biology, University of Padova, Padova, Italy 35100,1 Department of Cellular Biology, University of Georgia, Athens, Georgia 30602,2 Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York 148533

Received 28 July 2005/ Accepted 25 October 2005

We describe a novel metallothionein gene from Tetrahymena thermophila that has a strong copper-inducible promoter. This promoter can be turned on and off rapidly, making it a useful system for induction of ectopic gene expression in Tetrahymena and enhancing its applications in cell and molecular biology, as well as biotechnology.

* Corresponding author. Mailing address: Department of Biology, Via Ugo Bassi 58/B, 35100 Padova, Italy. Phone: 39-049-827-6310. Fax: 39-049-827-6300. E-mail: francesco.boldrin{at}unipd.it.

Eukaryotic Cell, February 2006, p. 422-425, Vol. 5, No. 2
1535-9778/06/$08.00+0     doi:10.1128/EC.5.2.422-425.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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Copyright © 2006 by the American Society for Microbiology.