Eukaryotic Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
EC.00287-05v1
5/12/2024    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cabral, M.
Right arrow Articles by Kuspa, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cabral, M.
Right arrow Articles by Kuspa, A.

 Previous Article  |  Next Article 

Eukaryotic Cell, December 2006, p. 2024-2032, Vol. 5, No. 12
1535-9778/06/$08.00+0     doi:10.1128/EC.00287-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Genetic Evidence that the Acyl Coenzyme A Binding Protein AcbA and the Serine Protease/ABC Transporter TagA Function Together in Dictyostelium discoideum Cell Differentiation{triangledown}

Matthew Cabral,1,2 Christophe Anjard,3 William F. Loomis,3 and Adam Kuspa1,2*

Departments of Molecular and Human Genetics,1 Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030,2 Center for Molecular Genetics, Division of Biological Sciences, University of California San Diego, La Jolla, California 920933

Received 7 September 2006/ Accepted 10 October 2006

The acyl coenzyme A (CoA) binding protein AcbA is cleaved to form a peptide (SDF-2) that coordinates spore encapsulation during the morphogenesis of Dictyostelium discoideum fruiting bodies. We present genetic evidence that the misspecification of cell types seen in mutants of the serine protease/ABC transporter TagA results from the loss of normal interactions with AcbA. Developmental phenotypes resulting from aberrant expression of the TagA protease domain, such as the formation of supernumerary tips on aggregates and the production of excess prestalk cells, are suppressed by null mutations in the acbA gene. Phenotypes resulting from the deletion of tagA, such as overexpression of the prestalk gene ecmB and the misexpression of the prespore gene cotB in stalk cells, are also observed in acbA mutants. Moreover, tagA– mutants fail to produce SDF-2 during fruiting body morphogenesis but are able to do so if they are stimulated with exogenous SDF-2. These results indicate that the developmental program depends on TagA and AcbA working in concert with each other during cell type differentiation and suggest that TagA is required for normal SDF-2 signaling during spore encapsulation.

* Corresponding author. Mailing address: Biochemistry and Molecular Biology, Baylor College of Medicine, Mail Station 356A, Houston, TX 77030. Phone: (713) 798-9278. Fax: (713) 798-4086. E-mail: akuspa{at}bcm.tmc.edu.

{triangledown} Published ahead of print on 20 October 2006.

Eukaryotic Cell, December 2006, p. 2024-2032, Vol. 5, No. 12
1535-9778/06/$08.00+0     doi:10.1128/EC.00287-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Appl. Environ. Microbiol. Infect. Immun. J. Bacteriol.
Mol. Cell Biol. Microbiol. Mol. Biol. Rev. ALL ASM JOURNALS
Copyright © 2006 by the American Society for Microbiology.