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Eukaryotic Cell, December 2006, p. 1980-1989, Vol. 5, No. 12
1535-9778/06/$08.00+0     doi:10.1128/EC.00272-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Reciprocal Nuclear Shuttling of Two Antagonizing Zn Finger Proteins Modulates Tup Family Corepressor Function To Repress Chromatin Remodeling

Kouji Hirota,^1* Charles S. Hoffman,² and Kunihiro Ohta^1*

Genetic System Regulation Laboratory, RIKEN (The Institute of Physical and Chemical Research), Discovery Research Institute, Wako-shi, Saitama 351-0198, Japan,¹ Biology Department, Boston College, Chestnut Hill, Massachusetts 02467²

Received 28 August 2006/ Accepted 26 September 2006

The Schizosaccharomyces pombe global corepressors Tup11 and Tup12, which are orthologs of Saccharomyces cerevisiae Tup1, are involved in glucose-dependent transcriptional repression and chromatin alteration of the fbp1⁺ gene. The fbp1⁺ promoter contains two regulatory elements, UAS1 and UAS2, one of which (UAS2) serves as a binding site for two antagonizing C₂H₂ Zn finger transcription factors, the Rst2 activator and the Scr1 repressor. In this study, we analyzed the role of Tup proteins and Scr1 in chromatin remodeling at fbp1⁺ during glucose repression. We found that Scr1, cooperating with Tup11 and Tup12, functions to maintain the chromatin of the fbp1⁺ promoter in a transcriptionally inactive state under glucose-rich conditions. Consistent with this notion, Scr1 is quickly exported from the nucleus to the cytoplasm at the initial stage of derepression, immediately after glucose starvation, at which time Rst2 is known to be imported into the nucleus. In addition, chromatin immunoprecipitation assays revealed a switching of Scr1 to Rst2 bound at UAS2 during glucose derepression. On the other hand, Tup11 and Tup12 persist in the nucleus and bind to the fbp1⁺ promoter under both derepressed and repressed conditions. These observations suggest that Tup1-like proteins recruited to the fbp1⁺ promoter are controlled by either of two antagonizing C₂H₂ Zn finger proteins. We propose that the actions of Tup11 and Tup12 are regulated by reciprocal nuclear shuttling of the two antagonizing Zn finger proteins in response to the extracellular glucose concentration. This notion provides new insights into the molecular mechanisms of the Tup family corepressors in gene regulation.

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* Corresponding author. Mailing address: Genetic System Regulation Laboratory, RIKEN (The Institute of Physical and Chemical Research), Discovery Research Institute, Wako-shi, Saitama 351-0198, Japan. Phone: 81-48-467-9277. Fax: 81-48-462-4691. E-mail for Kunihiro Ohta: kohta{at}riken.jp. E-mail for Kouji Hirota: khirota{at}riken.jp.

Published ahead of print on 6 October 2006.

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Eukaryotic Cell, December 2006, p. 1980-1989, Vol. 5, No. 12
1535-9778/06/$08.00+0     doi:10.1128/EC.00272-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

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