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Eukaryotic Cell, October 2006, p. 1760-1769, Vol. 5, No. 10
1535-9778/06/$08.00+0     doi:10.1128/EC.00159-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Cooperation between Reverse Transcriptase and Integrase during Reverse Transcription and Formation of the Preintegrative Complex of Ty1

Marcelle Wilhelm and F.-X. Wilhelm*

Institut de Biologie Moleculaire et Cellulaire, 15 Rue R. Descartes, 67084 Strasbourg, France

Received 1 June 2006/ Accepted 17 July 2006

Reverse transcriptase (RT) and integrase (IN) play a central role in the replication and transposition of retroelements. Increasing evidence suggests that the interaction between these two enzymes is functional and plays an important role in replication. In the yeast Saccharomyces cerevisiae retrotransposon Ty1, the interaction of IN with RT is critical for the formation of an active conformation of RT. We show here that the RT associated with VLPs is active only if it is in close interaction with IN. To probe the IN-RT cis-trans relationship, we have used a complementation assay based on coexpressing two transposons. We show that IN acts in cis to activate RT and that a functional integrase provided in trans is not able to complement replication and transposition defects of IN deletion or IN active-site mutant elements. Our data support a model in which IN not only interacts closely with RT during reverse transcription but also remains associated with RT during the formation of the preintegrative complex.

* Corresponding author. Mailing address: Institut de Biologie Moleculaire et Cellulaire, 15 Rue R. Descartes, 67084 Strasbourg, France. Phone: 33 3 88 41 70 06. Fax: 33 3 88 60 22 18. E-mail: wilhelm{at}ibmc.u-strasbg.fr.

Eukaryotic Cell, October 2006, p. 1760-1769, Vol. 5, No. 10
1535-9778/06/$08.00+0     doi:10.1128/EC.00159-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Moore, S. P., Garfinkel, D. J. (2009). Functional Analysis of N-Terminal Residues of Ty1 Integrase. J. Virol. 83: 9502-9511 [Abstract] [Full Text]  
  • Matsuda, E., Garfinkel, D. J. (2009). Posttranslational interference of Ty1 retrotransposition by antisense RNAs. Proc. Natl. Acad. Sci. USA 106: 15657-15662 [Abstract] [Full Text]  
  • Chatterjee, A. G., Leem, Y. E., Kelly, F. D., Levin, H. L. (2009). The Chromodomain of Tf1 Integrase Promotes Binding to cDNA and Mediates Target Site Selection. J. Virol. 83: 2675-2685 [Abstract] [Full Text]  


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