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Eukaryotic Cell, October 2006, p. 1713-1725, Vol. 5, No. 10
1535-9778/06/$08.00+0     doi:10.1128/EC.00152-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Modulation of Leishmania ABC Protein Gene Expression through Life Stages and among Drug-Resistant Parasites{dagger}

Philippe Leprohon, Danielle Légaré, Isabelle Girard, Barbara Papadopoulou, and Marc Ouellette*

Centre de Recherche en Infectiologie et Division de Microbiologie, Université Laval, Québec City, Québec, Canada

Received 26 May 2006/ Accepted 21 July 2006

The ATP-binding cassette (ABC) protein superfamily is one of the largest evolutionarily conserved families and is found in all kingdoms of life. The recent completion of the Leishmania genome sequence allowed us to analyze and classify its encoded ABC proteins. The complete sequence predicts a data set of 42 open reading frames (ORFs) coding for proteins belonging to the ABC superfamily, with representative members of every major subfamily (from ABCA to ABCH) commonly found in eukaryotes. Comparative analysis showed that the same ABC data set is found between Leishmania major and Leishmania infantum and that some orthologues are found in the genome of the related parasites Trypanosoma brucei and Trypanosoma cruzi. Customized DNA microarrays were made to assess ABC gene expression profiling throughout the two main Leishmania life stages. Two ABC genes (ABCA3 and ABCG3) are preferentially expressed in the amastigote stage, whereas one ABC gene (ABCF3) is more abundantly expressed in promastigotes. Microarray-based expression profiling experiments also revealed that three ABC genes (ABCA3, ABCC3, and ABCH1) are overexpressed in two independent antimony-resistant strains compared to the parental sensitive strain. All microarray results were confirmed by real-time reverse transcription-PCR assays. The present study provides a thorough phylogenic classification of the Leishmania ABC proteins and sets the basis for further functional studies on this important class of proteins.

* Corresponding author. Mailing address: Centre de Recherche en Infectiologie, 2705 Boul. Laurier, Québec, Québec G1V 4G2, Canada. Phone: 1-418-654-2705. Fax: 1-418-654-2715. E-mail: Marc.Ouellette{at}crchul.ulaval.ca.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

Eukaryotic Cell, October 2006, p. 1713-1725, Vol. 5, No. 10
1535-9778/06/$08.00+0     doi:10.1128/EC.00152-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Leprohon, P., Legare, D., Ouellette, M. (2009). Intracellular Localization of the ABCC Proteins of Leishmania and Their Role in Resistance to Antimonials. Antimicrob. Agents Chemother. 53: 2646-2649 [Abstract] [Full Text]  
  • Sanderson, L., Dogruel, M., Rodgers, J., De Koning, H. P., Thomas, S. A. (2009). Pentamidine Movement across the Murine Blood-Brain and Blood-Cerebrospinal Fluid Barriers: Effect of Trypanosome Infection, Combination Therapy, P-Glycoprotein, and Multidrug Resistance-Associated Protein. J. Pharmacol. Exp. Ther. 329: 967-977 [Abstract] [Full Text]  
  • Leprohon, P., Legare, D., Raymond, F., Madore, E., Hardiman, G., Corbeil, J., Ouellette, M. (2009). Gene expression modulation is associated with gene amplification, supernumerary chromosomes and chromosome loss in antimony-resistant Leishmania infantum. Nucleic Acids Res 37: 1387-1399 [Abstract] [Full Text]  
  • Castanys-Munoz, E., Perez-Victoria, J. M., Gamarro, F., Castanys, S. (2008). Characterization of an ABCG-Like Transporter from the Protozoan Parasite Leishmania with a Role in Drug Resistance and Transbilayer Lipid Movement. Antimicrob. Agents Chemother. 52: 3573-3579 [Abstract] [Full Text]  
  • Coelho, A. C., Messier, N., Ouellette, M., Cotrim, P. C. (2007). Role of the ABC Transporter PRP1 (ABCC7) in Pentamidine Resistance in Leishmania Amastigotes. Antimicrob. Agents Chemother. 51: 3030-3032 [Abstract] [Full Text]  


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