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 Previous Article

Eukaryotic Cell, January 2006, p. 212-215, Vol. 5, No. 1
1535-9778/06/$08.00+0     doi:10.1128/EC.5.1.212-215.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Gene Targeting in Aspergillus fumigatus by Homologous Recombination Is Facilitated in a Nonhomologous End- Joining-Deficient Genetic Background

Sven Krappmann,* Christoph Sasse, and Gerhard H. Braus

Institute of Microbiology & Genetics, Department of Molecular Microbiology and Genetics, Georg-August-University, Göttingen, Germany

Received 10 October 2005/ Accepted 7 November 2005

The akuA gene encoding the Ku70 component of the nonhomologous end-joining machinery was deleted in the opportunistic pathogen Aspergillus fumigatus. No obvious phenotype could be assessed for the corresponding mutant strain but relative frequencies of homologous recombination were increased as deduced from targeting the laccase-encoding abr2 gene.

* Corresponding author. Mailing address: Institute of Microbiology & Genetics, Department of Molecular Microbiology and Genetics, Grisebachstr. 8, D-37077 Göttingen, Germany. Phone: 49 551 39-3772. Fax: 49 551 39-3820. E-mail: skrappm{at}gwdg.de.

Eukaryotic Cell, January 2006, p. 212-215, Vol. 5, No. 1
1535-9778/06/$08.00+0     doi:10.1128/EC.5.1.212-215.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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