Eukaryotic Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by da Silva Ferreira, M. E.
Right arrow Articles by Goldman, G. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by da Silva Ferreira, M. E.
Right arrow Articles by Goldman, G. H.

 Previous Article  |  Next Article 

Eukaryotic Cell, January 2006, p. 207-211, Vol. 5, No. 1
1535-9778/06/$08.00+0     doi:10.1128/EC.5.1.207-211.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The akuBKU80 Mutant Deficient for Nonhomologous End Joining Is a Powerful Tool for Analyzing Pathogenicity in Aspergillus fumigatus

Márcia Eliana da Silva Ferreira,1 Marcia R. V. Z. Kress,1 Marcela Savoldi,1 Maria Helena S. Goldman,2 Albert Härtl,3 Thorsten Heinekamp,4 Axel A. Brakhage,4 and Gustavo H. Goldman1*

Faculdade de Ciências Farmacêuticas de Ribeirão Preto,1 Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil,2 Drug Testing Group,3 Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology, Hans-Knoell-Institut, Jena, Germany4

Received 10 October 2005/ Accepted 7 November 2005

To increase the frequency of homologous recombination, we inactivated the KU80 homologue in Aspergillus fumigatus (named akuBKU80). Homologous integration reached about 80% for both calcineurin A (calA) and polyketide synthase pksP (alb1) genes in the akuBKU80 mutant to 3 and 5%, respectively, when using a wild-type A. fumigatus strain. Deletion of akuBKU80 had no influence on pathogenicity in a low-dose murine infection model.

* Corresponding author. Mailing address: Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café S/N, CEP 14040-903, Ribeirão Preto, São Paulo, Brazil. Phone: 55-16-36024280/81. Fax: 55-16-36331092. E-mail address: ggoldman{at}usp.br.

Eukaryotic Cell, January 2006, p. 207-211, Vol. 5, No. 1
1535-9778/06/$08.00+0     doi:10.1128/EC.5.1.207-211.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Appl. Environ. Microbiol. Infect. Immun. J. Bacteriol.
Mol. Cell Biol. Microbiol. Mol. Biol. Rev. ALL ASM JOURNALS
Copyright © 2006 by the American Society for Microbiology.