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Eukaryotic Cell, January 2006, p. 207-211, Vol. 5, No. 1
1535-9778/06/$08.00+0     doi:10.1128/EC.5.1.207-211.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

The akuBKU80 Mutant Deficient for Nonhomologous End Joining Is a Powerful Tool for Analyzing Pathogenicity in Aspergillus fumigatus

Márcia Eliana da Silva Ferreira,1 Marcia R. V. Z. Kress,1 Marcela Savoldi,1 Maria Helena S. Goldman,2 Albert Härtl,3 Thorsten Heinekamp,4 Axel A. Brakhage,4 and Gustavo H. Goldman1*

Faculdade de Ciências Farmacêuticas de Ribeirão Preto,1 Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil,2 Drug Testing Group,3 Department of Molecular and Applied Microbiology, Leibniz Institute for Natural Product Research and Infection Biology, Hans-Knoell-Institut, Jena, Germany4

Received 10 October 2005/ Accepted 7 November 2005

To increase the frequency of homologous recombination, we inactivated the KU80 homologue in Aspergillus fumigatus (named akuBKU80). Homologous integration reached about 80% for both calcineurin A (calA) and polyketide synthase pksP (alb1) genes in the akuBKU80 mutant to 3 and 5%, respectively, when using a wild-type A. fumigatus strain. Deletion of akuBKU80 had no influence on pathogenicity in a low-dose murine infection model.

* Corresponding author. Mailing address: Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café S/N, CEP 14040-903, Ribeirão Preto, São Paulo, Brazil. Phone: 55-16-36024280/81. Fax: 55-16-36331092. E-mail address: ggoldman{at}usp.br.

Eukaryotic Cell, January 2006, p. 207-211, Vol. 5, No. 1
1535-9778/06/$08.00+0     doi:10.1128/EC.5.1.207-211.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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