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Eukaryotic Cell, January 2006, p. 203-206, Vol. 5, No. 1
1535-9778/06/$08.00+0     doi:10.1128/EC.5.1.203-206.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Heterologous Expression of an Entamoeba histolytica Chitin Synthase in Saccharomyces cerevisiae

Katrina L. Van Dellen,1,{dagger} Dorota A. Bulik,1,{dagger} Charles A. Specht,2 Phillips W. Robbins,1 and John C. Samuelson1*

Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine,1 Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 021182

Received 30 November 2004/ Accepted 11 January 2005

Chitin in the cyst wall of Entamoeba histolytica is made by two chitin synthases (Chs), one of which is unique (EhCHS-1) and one of which resembles those of insects and nematodes (EhCHS-2). EhCHS-1 is deposited chitin in the lateral wall of transformed Saccharomyces cerevisiae Chs mutants, independent of accessory proteins (Chs4p to Chs7p) required by yeast Chs3p.

* Corresponding author. Mailing address: Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, 715 Albany St., Evans 426, Boston, MA 02118. Phone: (617) 414-1054. Fax: (617) 414-1041. E-mail: jsamuels{at}bu.edu.

{dagger} K.L.V.D. and D.A.B. contributed equally to this work.

Eukaryotic Cell, January 2006, p. 203-206, Vol. 5, No. 1
1535-9778/06/$08.00+0     doi:10.1128/EC.5.1.203-206.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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Copyright © 2006 by the American Society for Microbiology.