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Eukaryotic Cell, January 2006, p. 148-154, Vol. 5, No. 1
1535-9778/06/$08.00+0     doi:10.1128/EC.5.1.148-154.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Pyruvate-Phosphate Dikinase of Oxymonads and Parabasalia and the Evolution of Pyrophosphate-Dependent Glycolysis in Anaerobic Eukaryotes{dagger}

Claudio H. Slamovits and Patrick J. Keeling*

Canadian Institute for Advanced Research, Botany Department, University of British Columbia, 3529-6270 University Boulevard, Vancouver, British Columbia V6T 1Z4, Canada

Received 29 September 2005/ Accepted 8 November 2005

In pyrophosphate-dependent glycolysis, the ATP/ADP-dependent enzymes phosphofructokinase (PFK) and pyruvate kinase are replaced by the pyrophosphate-dependent PFK and pyruvate phosphate dikinase (PPDK), respectively. This variant of glycolysis is widespread among bacteria, but it also occurs in a few parasitic anaerobic eukaryotes such as Giardia and Entamoeba spp. We sequenced two genes for PPDK from the amitochondriate oxymonad Streblomastix strix and found evidence for PPDK in Trichomonas vaginalis and other parabasalia, where this enzyme was thought to be absent. The Streblomastix and Giardia genes may be related to one another, but those of Entamoeba and perhaps Trichomonas are distinct and more closely related to bacterial homologues. These findings suggest that pyrophosphate-dependent glycolysis is more widespread in eukaryotes than previously thought, enzymes from the pathway coexists with ATP-dependent more often than previously thought and may be spread by lateral transfer of genes for pyrophosphate-dependent enzymes from bacteria.

* Corresponding author. Mailing address: Canadian Institute for Advanced Research, Botany Department, University of British Columbia, 3529-6270 University Boulevard, Vancouver, British Columbia V6T 1Z4, Canada. Phone: (604) 822-4906. Fax: (604) 822-6089. E-mail: pkeeling{at}interchange.ubc.ca.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

Eukaryotic Cell, January 2006, p. 148-154, Vol. 5, No. 1
1535-9778/06/$08.00+0     doi:10.1128/EC.5.1.148-154.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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