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Eukaryotic Cell, September 2005, p. 1583-1590, Vol. 4, No. 9
1535-9778/05/$08.00+0     doi:10.1128/EC.4.9.1583-1590.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Functional Conservation and Specialization among Eukaryotic Anti-Silencing Function 1 Histone Chaperones

Beth A. Tamburini, Joshua J. Carson, Melissa W. Adkins, and Jessica K. Tyler*

Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Aurora, Colorado 80010

Received 17 May 2005/ Accepted 24 June 2005

Chromatin disassembly and reassembly, mediated by histone chaperones such as anti-silencing function 1 (Asf1), are likely to accompany all nuclear processes that occur on the DNA template. In order to gain insight into the functional conservation of Asf1 across eukaryotes, we have replaced the budding yeast Asf1 protein with Drosophila Asf1 (dAsf1) or either of the two human Asf1 (hAsf1a and hAsf1b) counterparts. We found that hAsf1b is best able to rescue the growth defect of Saccharomyces cerevisiae lacking Asf1. Moreover, dAsf1 and hAsf1b but not hAsf1a can replace the role of yeast Asf1 in protecting against replicational stress and activating the PHO5 gene, while only hAsf1a can replace the role of Asf1 in protecting against double-stranded-DNA-damaging agents. Furthermore, it appears that the interaction between Asf1 and the DNA damage checkpoint protein Rad53 is not required for Asf1's role in maintaining genomic integrity. In addition to indicating the functional conservation of the Asf1 proteins across species, these studies suggest distinct roles for the two human Asf1 proteins.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Genetics, UCHSC, Mail Stop 8101, P. O. Box 6511, Aurora CO 80045. Phone: 303 724 3224. Fax: 303 724 3221. E-mail: Jessica.tyler{at}uchsc.edu.

Eukaryotic Cell, September 2005, p. 1583-1590, Vol. 4, No. 9
1535-9778/05/$08.00+0     doi:10.1128/EC.4.9.1583-1590.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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