This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Fagundes, M. R. v. Z. K. Articles by Goldman, G. H. Search for Related Content PubMed PubMed Citation Articles by Fagundes, M. R. v. Z. K. Articles by Goldman, G. H.

Aspergillus nidulans uvsB^ATR and scaA^NBS1 Genes Show Genetic Interactions during Recovery from Replication Stress and DNA Damage

Marcia Regina von Zeska Kress Fagundes,^1, Camile P. Semighini,^2, Iran Malavazi,¹ Marcela Savoldi,¹ Joel Fernandes de Lima,¹ Maria Helena de Souza Goldman,³ Steven D. Harris,² and Gustavo Henrique Goldman^1*

Faculdade de Ciências Farmacêuticas de Ribeirão Preto,¹ Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil,³ Plant Science Initiative, University of Nebraska, N234 Beadle Center, Lincoln, Nebraska 68588-0660²

Received 22 February 2005/ Accepted 19 April 2005

The ATM/ATR kinases and the Mre11 (Mre11-Rad50-Nbs1) protein complex are central players in the cellular DNA damage response. Here we characterize possible interactions between Aspergillus nidulans uvsB^ATR and the Mre11 complex (scaA^NBS1). We demonstrate that there is an epistatic relationship between uvsB^ATR, the homolog of the ATR/MEC1 gene, and scaA^NBS1, the homolog of the NBS1/XRS2 gene, for both repair and checkpoint functions and that correct ScaA^NBS1 expression during recovery from replication stress depends on uvsB^ATR. In addition, we also show that the formation of UvsC foci during recovery from replication stress is dependent on both uvsB^ATR and scaA^NBS1 function. Furthermore, ScaA^NBS1 is also dependent on uvsB^ATR for nuclear focus formation upon the induction of DNA double-strand breaks by phleomycin. Our results highlight the extensive genetic interactions between UvsB and the Mre11 complex that are required for S-phase progression and recovery from DNA damage.

Both authors contributed equally to this work.

This article has been cited by other articles:

• Malavazi, I., Lima, J. F., de Castro, P. A., Savoldi, M., de Souza Goldman, M. H., Goldman, G. H. (2008). Genetic Interactions of the Aspergillus nidulans atmAATM Homolog With Different Components of the DNA Damage Response Pathway. Genetics 178: 675-691 [Abstract] [Full Text]  
• De Souza, C. P. C., Hashmi, S. B., Horn, K. P., Osmani, S. A. (2006). A Point Mutation in the Aspergillus nidulans sonBNup98 Nuclear Pore Complex Gene Causes Conditional DNA Damage Sensitivity. Genetics 174: 1881-1893 [Abstract] [Full Text]  
• Malavazi, I., Savoldi, M., Di Mauro, S. M. Z., Menck, C. F. M., Harris, S. D., Goldman, M. H. d. S., Goldman, G. H. (2006). Transcriptome Analysis of Aspergillus nidulans Exposed to Camptothecin-Induced DNA Damage.. Eukaryot Cell 5: 1688-1704 [Abstract] [Full Text]  
• Malavazi, I., Semighini, C. P., von Zeska Kress, M. R., Harris, S. D., Goldman, G. H. (2006). Regulation of Hyphal Morphogenesis and the DNA Damage Response by the Aspergillus nidulans ATM Homolog AtmA. Genetics 173: 99-109 [Abstract] [Full Text]  
• Semighini, C. P., Savoldi, M., Goldman, G. H., Harris, S. D. (2006). Functional Characterization of the Putative Aspergillus nidulans Poly(ADP-Ribose) Polymerase Homolog PrpA. Genetics 173: 87-98 [Abstract] [Full Text]