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Eukaryotic Cell, July 2005, p. 1239-1252, Vol. 4, No. 7
1535-9778/05/$08.00+0     doi:10.1128/EC.4.7.1239-1252.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Aspergillus nidulans uvsBATR and scaANBS1 Genes Show Genetic Interactions during Recovery from Replication Stress and DNA Damage

Marcia Regina von Zeska Kress Fagundes,1,{dagger} Camile P. Semighini,2,{dagger} Iran Malavazi,1 Marcela Savoldi,1 Joel Fernandes de Lima,1 Maria Helena de Souza Goldman,3 Steven D. Harris,2 and Gustavo Henrique Goldman1*

Faculdade de Ciências Farmacêuticas de Ribeirão Preto,1 Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil,3 Plant Science Initiative, University of Nebraska, N234 Beadle Center, Lincoln, Nebraska 68588-06602

Received 22 February 2005/ Accepted 19 April 2005

The ATM/ATR kinases and the Mre11 (Mre11-Rad50-Nbs1) protein complex are central players in the cellular DNA damage response. Here we characterize possible interactions between Aspergillus nidulans uvsBATR and the Mre11 complex (scaANBS1). We demonstrate that there is an epistatic relationship between uvsBATR, the homolog of the ATR/MEC1 gene, and scaANBS1, the homolog of the NBS1/XRS2 gene, for both repair and checkpoint functions and that correct ScaANBS1 expression during recovery from replication stress depends on uvsBATR. In addition, we also show that the formation of UvsC foci during recovery from replication stress is dependent on both uvsBATR and scaANBS1 function. Furthermore, ScaANBS1 is also dependent on uvsBATR for nuclear focus formation upon the induction of DNA double-strand breaks by phleomycin. Our results highlight the extensive genetic interactions between UvsB and the Mre11 complex that are required for S-phase progression and recovery from DNA damage.

* Corresponding author. Mailing address: Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Av. do Café S/N, CEP 14040-903, Ribeirão Preto, São Paulo, Brazil. Phone: 55-16-6024280/81. Fax: 55-16-6331092. E-mail: ggoldman{at}usp.br.

{dagger} Both authors contributed equally to this work.

Eukaryotic Cell, July 2005, p. 1239-1252, Vol. 4, No. 7
1535-9778/05/$08.00+0     doi:10.1128/EC.4.7.1239-1252.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Malavazi, I., Lima, J. F., de Castro, P. A., Savoldi, M., de Souza Goldman, M. H., Goldman, G. H. (2008). Genetic Interactions of the Aspergillus nidulans atmAATM Homolog With Different Components of the DNA Damage Response Pathway. Genetics 178: 675-691 [Abstract] [Full Text]  
  • De Souza, C. P. C., Hashmi, S. B., Horn, K. P., Osmani, S. A. (2006). A Point Mutation in the Aspergillus nidulans sonBNup98 Nuclear Pore Complex Gene Causes Conditional DNA Damage Sensitivity. Genetics 174: 1881-1893 [Abstract] [Full Text]  
  • Malavazi, I., Savoldi, M., Di Mauro, S. M. Z., Menck, C. F. M., Harris, S. D., Goldman, M. H. d. S., Goldman, G. H. (2006). Transcriptome Analysis of Aspergillus nidulans Exposed to Camptothecin-Induced DNA Damage.. Eukaryot Cell 5: 1688-1704 [Abstract] [Full Text]  
  • Malavazi, I., Semighini, C. P., von Zeska Kress, M. R., Harris, S. D., Goldman, G. H. (2006). Regulation of Hyphal Morphogenesis and the DNA Damage Response by the Aspergillus nidulans ATM Homolog AtmA. Genetics 173: 99-109 [Abstract] [Full Text]  
  • Semighini, C. P., Savoldi, M., Goldman, G. H., Harris, S. D. (2006). Functional Characterization of the Putative Aspergillus nidulans Poly(ADP-Ribose) Polymerase Homolog PrpA. Genetics 173: 87-98 [Abstract] [Full Text]  


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