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Eukaryotic Cell, July 2005, p. 1203-1210, Vol. 4, No. 7
1535-9778/05/$08.00+0     doi:10.1128/EC.4.7.1203-1210.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Release from Quorum-Sensing Molecules Triggers Hyphal Formation during Candida albicans Resumption of Growth{dagger}

Brice Enjalbert1* and Malcolm Whiteway2

Aberdeen Fungal Group, School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, United Kingdom,1 Eukaryotic Genetics Group, NRC Biotechnology Research Institute, 6100 Royalmount Avenue, Montreal, Quebec, Canada H4P 2R22

Received 20 January 2005/ Accepted 27 April 2005

Candida albicans is a pathogenic fungus able to change morphology in response to variations in its growth environment. Simple inoculation of stationary cells into fresh medium at 37°C, without any other manipulations, appears to be a powerful but transient inducer of hyphal formation; this process also plays a significant role in classical serum induction of hyphal formation. The mechanism appears to involve the release of hyphal repression caused by quorum-sensing molecules in the growth medium of stationary-phase cells, and farnesol has a strong but incomplete role in this process. We used DNA microarray technology to study both the resumption of growth of Candida albicans cells and molecular regulation involving farnesol. Maintaining farnesol in the culture medium during the resumption of growth both delays and reduces the induction of hypha-related genes yet triggers expression of genes encoding drug efflux components. The persistence of farnesol also prevents the repression of histone genes during hyphal growth and affects the expression of putative or demonstrated morphogenesis-regulating cyclin genes, such as HGC1, CLN3, and PCL2. The results suggest a model explaining the triggering of hyphae in the host based on quorum-sensing molecules.

* Corresponding author. Mailing address: Aberdeen Fungal Group, School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, United Kingdom. Phone: (44)-1224-555882. Fax: (44)-1224-555844. E-mail: brice.enjalbert{at}abdn.ac.uk.

{dagger} This is National Research Council publication number 47468.

Eukaryotic Cell, July 2005, p. 1203-1210, Vol. 4, No. 7
1535-9778/05/$08.00+0     doi:10.1128/EC.4.7.1203-1210.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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