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Eukaryotic Cell, July 2005, p. 1191-1202, Vol. 4, No. 7
1535-9778/05/$08.00+0     doi:10.1128/EC.4.7.1191-1202.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Cell Cycle Dynamics and Quorum Sensing in Candida albicans Chlamydospores Are Distinct from Budding and Hyphal Growth

Stephen W. Martin,1,{dagger} Lois M. Douglas,2 and James B. Konopka2*

Program in Molecular and Cellular Biology,1 Department of Molecular Genetics and Microbiology, State University of New York, Stony Brook, New York 11794-52222

Received 4 April 2005/ Accepted 25 April 2005

The regulation of morphogenesis in the human fungal pathogen Candida albicans is under investigation to better understand how the switch between budding and hyphal growth is linked to virulence. Therefore, in this study we examined the ability of C. albicans to undergo a distinct type of morphogenesis to form large thick-walled chlamydospores whose role in infection is unclear, but they act as a resting form in other species. During chlamydospore morphogenesis, cells switch to filamentous growth and then develop elongated suspensor cells that give rise to chlamydospores. These filamentous cells were distinct from true hyphae in that they were wider and were not inhibited by the quorum-sensing factor farnesol. Instead, farnesol increased chlamydospore production, indicating that quorum sensing can also have a positive role. Nuclear division did not occur across the necks of chlamydospores, as it does in budding. Interestingly, nuclei divided within the suspensor cells, and then one daughter nucleus subsequently migrated into the chlamydospore. Septins were not detected near mitotic nuclei but were localized at chlamydospore necks. At later stages, septins localized throughout the chlamydospore plasma membrane and appeared to form long filamentous structures. Deletion of the CDC10 or CDC11 septins caused greater curvature of cells growing in a filamentous manner and morphological defects in suspensor cells and chlamydospores. These studies identify aspects of chlamydospore morphogenesis that are distinct from bud and hyphal morphogenesis.

* Corresponding author. Mailing address: Department of Molecular Genetics and Microbiology, State University of New York, Stony Brook, NY 11794-5222. Phone: (631) 632-8715. Fax: (631) 632-9797. E-mail: james.konopka{at}sunysb.edu.

{dagger} Present address: Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom.

Eukaryotic Cell, July 2005, p. 1191-1202, Vol. 4, No. 7
1535-9778/05/$08.00+0     doi:10.1128/EC.4.7.1191-1202.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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