This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Veses, V.
Right arrow Articles by Martínez, J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Veses, V.
Right arrow Articles by Martínez, J. P.

 Previous Article  |  Next Article 

Eukaryotic Cell, June 2005, p. 1088-1101, Vol. 4, No. 6
1535-9778/05/$08.00+0     doi:10.1128/EC.4.6.1088-1101.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

ABG1, a Novel and Essential Candida albicans Gene Encoding a Vacuolar Protein Involved in Cytokinesis and Hyphal Branching

Verónica Veses,1 Manuel Casanova,1 Amelia Murgui,2 Ángel Domínguez,3* Neil A. R. Gow,4 and José P. Martínez1*

Departamento de Microbiología y Ecologí, Facultad de Farmacia, Universitat de València, 46100-Burjasot, Valencia, Spain,1 Departamento de Bioquímica y Biología Molecular, Facultad de Farmacia, Universitat de València, 46100-Burjasot, Valencia, Spain,2 Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain,3 Department of Molecular and Cell Biology, Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, United Kingdom4

Received 1 March 2005/ Accepted 19 April 2005

Immunoscreening of a Candida albicans expression library resulted in the isolation of a novel gene encoding a 32.9-kDa polypeptide (288 amino acids), with 27.7% homology to the product of Saccharomyces cerevisiae YGR106c, a putative vacuolar protein. Heterozygous mutants in this gene displayed an altered budding growth pattern, characterized by the formation of chains of buds, decreasingly in size towards the apex, without separation of the daughter buds. Consequently, this gene was designated ABG1. A conditional mutant for ABG1 with the remaining allele under the control of the MET3 promoter did not grow in the presence of methionine and cysteine, demonstrating that ABG1 was essential for viability. Western analysis revealed the presence of a major 32.9-kDa band, mainly in a particulate fraction (P40) enriched in vacuoles, and tagging with green fluorescent protein confirmed that Abg1p localized to the vacuole. Vacuole inheritance has been linked to the regulation of branching frequency in C. albicans. Under repressing conditions, the conditional mutant had an increased frequency of branching under hyphal inducing conditions and an altered sensitivity to substances that interfered with cell wall assembly. Repression of ABG1 in the conditional mutant strain caused disturbance of normal size and number of vacuoles both in yeast and mycelial cells and also in the asymmetric vacuole inheritance associated with the characteristic pattern of germ tubes and branching in C. albicans. These observations indicate that ABG1 plays a key role in vacuole biogenesis, cytokinesis, and hyphal branching.

* Corresponding author. Mailing address for José P. Martínez: Departamento de Microbiología y Ecologí, Facultad de Farmacia, Universitat de València, 46100-Burjasot, Valencia, Spain. Phone: (34) 963544770. Fax: (34) 963544299. E-mail: jose.pedro.martinez{at}uv.es. Mailing address for Ángel Domínguez: Departamento de Microbiología y Genética, Edificio Departamental, Plaza de los Doctores de la Reina s/n, Universidad de Salamanca, 37007 Salamanca, Spain. Phone: (34) 923294677. Fax: (34) 923224876. E-mail: ado{at}usal.es.

Eukaryotic Cell, June 2005, p. 1088-1101, Vol. 4, No. 6
1535-9778/05/$08.00+0     doi:10.1128/EC.4.6.1088-1101.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ryan, M., Graham, L. A., Stevens, T. H. (2008). Voa1p Functions in V-ATPase Assembly in the Yeast Endoplasmic Reticulum. Mol. Biol. Cell 19: 5131-5142 [Abstract] [Full Text]  
  • Palmer, G. E., Kelly, M. N., Sturtevant, J. E. (2007). Autophagy in the pathogen Candida albicans. Microbiology 153: 51-58 [Abstract] [Full Text]  
  • Barelle, C. J., Richard, M. L., Gaillardin, C., Gow, N. A. R., Brown, A. J. P. (2006). Candida albicans VAC8 Is Required for Vacuolar Inheritance and Normal Hyphal Branching. Eukaryot Cell 5: 359-367 [Abstract] [Full Text]  
  • Palmer, G. E., Kelly, M. N., Sturtevant, J. E. (2005). The Candida albicans Vacuole Is Required for Differentiation and Efficient Macrophage Killing. Eukaryot Cell 4: 1677-1686 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»