This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Barwell, K. J.
Right arrow Articles by Mitchell, A. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Barwell, K. J.
Right arrow Articles by Mitchell, A. P.

 Previous Article  |  Next Article 

Eukaryotic Cell, May 2005, p. 890-899, Vol. 4, No. 5
1535-9778/05/$08.00+0     doi:10.1128/EC.4.5.890-899.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Relationship of DFG16 to the Rim101p pH Response Pathway in Saccharomyces cerevisiae and Candida albicans{dagger}

Karen J. Barwell,1,2 Jacob H. Boysen,3 Wenjie Xu,3 and Aaron P. Mitchell1,2,3*

Department of Microbiology,1 Institute of Cancer Research,2 Integrated Program in Cellular, Molecular, and Biophysical Studies, Columbia University, New York, New York 100323

Received 13 February 2005/ Accepted 24 February 2005

Many fungal pH responses depend upon conserved Rim101p/PacC transcription factors, which are activated by C-terminal proteolytic processing. The means by which environmental pH is sensed by this pathway are not known. Here, we report a screen of the Saccharomyces cerevisiae viable deletion mutant library that has yielded a new gene required for processed Rim101p accumulation, DFG16. An S. cerevisiae dfg16{Delta} mutant expresses Rim101p-repressed genes at elevated levels. In addition, Candida albicans dfg16{Delta}/dfg16{Delta} mutants are defective in alkaline pH-induced filamentation, and their defect is suppressed by expression of truncated Rim101-405p. Thus, Dfg16p is a functionally conserved Rim101p pathway member. Many proteins required for processed Rim101p accumulation are members of the ESCRT complex, which functions in the formation of multivesicular bodies (MVBs). Staining with the dye FM4-64 indicates that the S. cerevisiae dfg16{Delta} mutant does not have an MVB defect. We find that two transcripts, PRY1 and ASN1, respond to mutations that affect both the Rim101p and MVB pathways but not to mutations that affect only one pathway. The S. cerevisiae dfg16{Delta} mutation does not affect PRY1 and ASN1 expression, thus confirming that Dfg16p function is restricted to the Rim101p pathway. Dfg16p is homologous to Aspergillus nidulans PalH, a component of the well-characterized PacC processing pathway. We verify that the previously recognized PalH homolog, Rim21p, also functions in the S. cerevisiae Rim101p pathway. Dfg16p is predicted to have seven membrane-spanning segments and a long hydrophilic C-terminal region, as expected if Dfg16p were a G-protein-coupled receptor.

* Corresponding author. Mailing address: Department of Microbiology, Columbia University, 701 West 168th Street, New York, NY 10032. Phone: (212) 305-8251. Fax: (212) 305-1741. E-mail: apm4{at}columbia.edu.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

Eukaryotic Cell, May 2005, p. 890-899, Vol. 4, No. 5
1535-9778/05/$08.00+0     doi:10.1128/EC.4.5.890-899.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zhang, F., Gaur, N. A., Hasek, J., Kim, S.-j., Qiu, H., Swanson, M. J., Hinnebusch, A. G. (2008). Disrupting Vesicular Trafficking at the Endosome Attenuates Transcriptional Activation by Gcn4. Mol. Cell. Biol. 28: 6796-6818 [Abstract] [Full Text]  
  • Blanchin-Roland, S., Da Costa, G., Gaillardin, C. (2008). Ambient pH signalling in the yeast Yarrowia lipolytica involves YlRim23p/PalC, which interacts with Snf7p/Vps32p, but does not require the long C terminus of YlRim9p/PalI. Microbiology 154: 1668-1676 [Abstract] [Full Text]  
  • Mitchell, A. P. (2008). A VAST staging area for regulatory proteins. Proc. Natl. Acad. Sci. USA 105: 7111-7112 [Full Text]  
  • Ikeda, M., Kihara, A., Denpoh, A., Igarashi, Y. (2008). The Rim101 Pathway Is Involved in Rsb1 Expression Induced by Altered Lipid Asymmetry. Mol. Biol. Cell 19: 1922-1931 [Abstract] [Full Text]  
  • Calcagno-Pizarelli, A. M., Negrete-Urtasun, S., Denison, S. H., Rudnicka, J. D., Bussink, H.-J., Munera-Huertas, T., Stanton, L., Hervas-Aguilar, A., Espeso, E. A., Tilburn, J., Arst, H. N. Jr., Penalva, M. A. (2007). Establishment of the Ambient pH Signaling Complex in Aspergillus nidulans: PalI Assists Plasma Membrane Localization of PalH. Eukaryot Cell 6: 2365-2375 [Abstract] [Full Text]  
  • Biswas, S., Van Dijck, P., Datta, A. (2007). Environmental Sensing and Signal Transduction Pathways Regulating Morphopathogenic Determinants of Candida albicans. Microbiol. Mol. Biol. Rev. 71: 348-376 [Abstract] [Full Text]  
  • Penas, M. M., Hervas-Aguilar, A., Munera-Huertas, T., Reoyo, E., Penalva, M. A., Arst, H. N. Jr., Tilburn, J. (2007). Further Characterization of the Signaling Proteolysis Step in the Aspergillus nidulans pH Signal Transduction Pathway. Eukaryot Cell 6: 960-970 [Abstract] [Full Text]  
  • Morohashi, N., Yamamoto, Y., Kuwana, S., Morita, W., Shindo, H., Mitchell, A. P., Shimizu, M. (2006). Effect of Sequence-Directed Nucleosome Disruption on Cell-Type-Specific Repression by {alpha}2/Mcm1 in the Yeast Genome. Eukaryot Cell 5: 1925-1933 [Abstract] [Full Text]  
  • Boysen, J. H., Mitchell, A. P. (2006). Control of Bro1-Domain Protein Rim20 Localization by External pH, ESCRT Machinery, and the Saccharomyces cerevisiae Rim101 Pathway. Mol. Biol. Cell 17: 1344-1353 [Abstract] [Full Text]  
  • Blanchin-Roland, S., Costa, G. D., Gaillardin, C. (2005). ESCRT-I components of the endocytic machinery are required for Rim101-dependent ambient pH regulation in the yeast Yarrowia lipolytica. Microbiology 151: 3627-3637 [Abstract] [Full Text]  
  • Herranz, S., Rodriguez, J. M., Bussink, H.-J., Sanchez-Ferrero, J. C., Arst, H. N. Jr., Penalva, M. A., Vincent, O. (2005). Arrestin-related proteins mediate pH signaling in fungi. Proc. Natl. Acad. Sci. USA 102: 12141-12146 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»