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Eukaryotic Cell, May 2005, p. 861-866, Vol. 4, No. 5
1535-9778/05/$08.00+0     doi:10.1128/EC.4.5.861-866.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Role of Tos3, a Snf1 Protein Kinase Kinase, during Growth of Saccharomyces cerevisiae on Nonfermentable Carbon Sources

Myoung-Dong Kim, Seung-Pyo Hong, and Marian Carlson*

Departments of Genetics and Development and Microbiology, Columbia University, New York, NY

Received 11 February 2005/ Accepted 21 March 2005

In Saccharomyces cerevisiae, Snf1 protein kinase of the Snf1/AMP-activated protein kinase family is required for growth on nonfermentable carbon sources and nonpreferred sugars. Three kinases, Pak1, Elm1, and Tos3, activate Snf1 by phosphorylation of its activation-loop threonine, and the absence of all three causes the Snf– phenotype. No phenotype has previously been reported for the tos3{Delta} single mutation. We show here that, when cells are grown on glycerol-ethanol, tos3{Delta} reduces growth rate, Snf1 catalytic activity, and activation of the Snf1-dependent carbon source-responsive element (CSRE) in the promoters of gluconeogenic genes. In contrast, tos3{Delta} did not significantly affect Snf1 catalytic activity or CSRE function during abrupt glucose depletion, indicating that Tos3 has a more substantial role in activating Snf1 protein kinase during growth on a nonfermentable carbon source than during acute carbon stress. We also report that Tos3 is localized in the cytosol during growth in either glucose or glycerol-ethanol. These findings lend support to the idea that the Snf1 protein kinase kinases make different contributions to cellular regulation under different growth conditions.

* Corresponding author. Mailing address: 701 W. 168th Street, HSC922, New York, NY 10032. Phone: (212) 305-6314. Fax: (212) 305-1741. E-mail: mbc1{at}columbia.edu.

Eukaryotic Cell, May 2005, p. 861-866, Vol. 4, No. 5
1535-9778/05/$08.00+0     doi:10.1128/EC.4.5.861-866.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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